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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 4

sp. nov., isolated from commercial soil No Access

Abstract

A novel bacterial strain, designated DS6, was isolated from the commercial soil for biofertilization and characterized as a novel species within the phylum. The morphological and biochemical analysis revealed that strain DS6 is a Gram-positive, rod-shaped bacterium with pili, forming ivory colonies on R2A agar after 3 days of incubation at 25 °C. The strain shows a broad growth pH range of 4–9, with an optimum pH of 8, and can tolerate temperatures between 20 and 45 °C, optimally growing at 37 °C. Salinity tolerance ranges from 0% to 5%, with optimal growth occurring at 0–1%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DS6 is closely related to KCCM 4231 (96.4%), KCTC 19471 (96.3%), KCTC 49117 (96.3%) and KCTC 39594 (96.2%). Biochemical tests showed that the strain is oxidase negative and catalase positive. The major fatty acids were C iso (26.85%) and C 10-methyl (15.9%). The major respiratory quinone was MK-8 (H4) and the strain contained ll-2,6-diaminopimelic acid as the cell-wall diamino acid. The major polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phospholipids, phosphatidylinositolmannosides and unknown lipids. The DNA G+C mol% of genomic DNA is 71.3. Based on its distinct phylogenetic, phenotypic and biochemical characteristics, strain DS6 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS6 (KEMB 22008=KCTC 59264=JCM 37205).

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Keyword(s): Actinomycetota , Nocardioides , plant growth promoting , soil bacterium and taxonomy
Funding
This study was supported by the:
  • Ministry of Trade, Industry and Energy (Award 20025773)
    • Principal Award Recipient: Sang-SeobLee
  • National Research Foundation (Award NRF-2022M3H9A1085063)
    • Principal Award Recipient: Sang-SeobLee
© 2025 The Authors
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2026-02-18

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Nocardioides eburneus sp. nov., isolated from commercial soil
Int J Syst Evol Microbiol 75, 006743 (2025); https://doi.org/10.1099/ijsem.0.006743
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