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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 4

gen. nov., sp. nov., a deep-sea alkaliphilic cellulolytic bacterium isolated from an endemic ascidian Open Access

Abstract

The strain TOYAMA8 is a deep-sea alkaliphilic cellulolytic bacterium isolated from a slurry-adhered epiphytic site of . Cells of this strain are Gram-negative, aerobic, curved rods or spirilla, motile with monopolar flagella, and grow on cellulose as the sole carbon source. Compared to other closely related species, this bacterium is characterized by a large number of cellulase genes. Strain TOYAMA8 showed alkaliphilic growth within the pH range 7.5–9.0. The major cellular fatty acids were C ω7, C, C and C ω7. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipids and aminolipids. A major respiratory lipoquinone was Q-9. Phylogenomic analysis using the 16S rRNA gene and whole-genome sequence data showed that the strain is related to the families , and . The values of 16S rRNA gene sequence similarity, amino acid identity and percentage of conserved proteins between the strain TOYAMA8 and related species were low, with maximum values of 90.6, 48.1 and 34.6%, respectively. These results, together with differences in phenotypic and biochemical characteristics, indicate that the new isolate TOYAMA8 represents a novel genus and species, for which the name gen. nov., sp. nov., is proposed. The type strain is TOYAMA8 (JCM 31119=DSM 114864).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cellulose degradation , deep-sea , Gynuellaceae , Oceanospirillales , Pseudomonadales and Saccharospirillaceae
Funding
This study was supported by the:
  • Nagase Science Technology Foundation
    • Principal Award Recipient: ShigeruDeguchi
  • Core Research for Evolutional Science and Technology (Award MJCR21L4)
    • Principal Award Recipient: ShigeruDeguchi
  • Japan Society for the Promotion of Science (Award 23K14000, 19K15956)
    • Principal Award Recipient: MikakoTachioka
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2025-04-03
2025-12-15

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Marinicellulosiphila megalodicopiae gen. nov., sp. nov., a deep-sea alkaliphilic cellulolytic bacterium isolated from an endemic ascidian Megalodicopia hians
Int J Syst Evol Microbiol 75, 006742 (2025); https://doi.org/10.1099/ijsem.0.006742
/content/journal/ijsem/10.1099/ijsem.0.006742
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