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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 4

sp. nov., isolated from lead–zinc mine tailing No Access

Abstract

A strain with aerial hyphae presented, designated KC 131, was isolated from lead–zinc mine tailings, Lanping, Yunnan province, Southwest China. The strain was Gram-stain-positive, acid-alcohol-fast, aerobic and non-motile. Based on 16S rRNA gene sequence and phylogeny analysis, strains NBRC 108245 (98.4%), YIM 30243 (98.4%) and CICC 11023 (98.2%) were the most closely related species of strain KC 131, and the four strains were in one cluster. The -diaminopimelic acid, arabinose and galactose were detected in the whole-cell hydrolysates. The mycolic acids were presented, and the major fatty acids (>5%) were C (30.0%), C ω9c (12.5%), C (7.5%), 10-methyl C (27.3%) and summed feature 3 (C ω7c and/or C ω6c; 12.4%). The menaquinone was MK-8 (H4, ω-cyclo), and the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. All these features were consistent with the genus . Digital DNA–DNA hybridization values between strain KC 131 and NBRC 108245, DSM 44684 and CICC 11023 were 23.8%, 20.9% and 23.5%, respectively. The average nucleotide identity values between strain KC 131 and the three strains were 80.0%, 76.5% and 80.0%, respectively. In addition, some physiological and biochemical characteristics were also different. Thus, strain KC 131 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KC 131 (=CGMCC 1.19493=JCM 35916).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinomycetota , new taxa and Nocardia
Funding
This study was supported by the:
  • Major Science and Technology Projects in Yunnan Province (Award 202202AE090015)
    • Principle Award Recipient: Sheng-GuangXu
  • YNCUB (Award 2024KF007)
    • Principle Award Recipient: Yan-RuCao
  • Applied Basic Research Foundation of Yunnan Province (Award 202301AT070051)
    • Principle Award Recipient: Yan-RuCao
  • Joint Special Project of Universities in Yunnan Province (Award 202401BA070001-113)
    • Principle Award Recipient: XiuChen
  • Joint Special Project of Universities in Yunnan Province (Award 202401BA070001-003)
    • Principle Award Recipient: Yan-RuCao
© 2025 The Authors
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Nocardia arseniciresistens sp. nov., isolated from lead–zinc mine tailing
Int J Syst Evol Microbiol 75, 006738 (2025); https://doi.org/10.1099/ijsem.0.006738
/content/journal/ijsem/10.1099/ijsem.0.006738
/content/journal/ijsem/10.1099/ijsem.0.006738
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