Skip to content
1887

Abstract

is a perennial herbaceous plant with high genetic diversity, which is mainly distributed in the southwest of Yunnan. During our investigation of the diversity of seed endophytic fungi of , 157 strains of fungi were isolated; among them, 50 strains were identified as spp. based on internal transcribed spacer (ITS) sequences. Then, two additional loci, and , were sequenced for all strains of . Based on combined ITS, and sequence analyses, these strains were further identified to species level, including 15 known species and a new, so far undescribed, species. Based on combined morphological characteristics and phylogenetic analyses, one new species is introduced under the name . Detailed descriptions, illustrations and phylogenetic analyses of the new species are provided in this study.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32370017)
    • Principal Award Recipient: Ze-FenYu
  • National Natural Science Foundation of China (Award 32170017)
    • Principal Award Recipient: Ze-FenYu
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.006730
2025-03-28
2026-03-14

Metrics

Loading full text...

Full text loading...

References

  1. de Bary A. Morphology and physiology of fungi, lichens, and myxomycetes in Hofmeister’s handbook of physiological botany. leipzig: Wilhelm Engelmann; 1866
  2. Petrini O. Fungal endophytes of tree leaves. In Andrews JH. eds Microb Ecol Leaves New York, NY: Springer; pp 1991–197 [View Article]
    [Google Scholar]
  3. Wang ZS, Li N, Wang WP, Liu Y. Research progress in endophytic bacteria in rice seeds. Biotechnolo Bull 2022; 38:236
    [Google Scholar]
  4. Cleary M, Oskay F, Doğmuş HT, Lehtijärvi A, Woodward S et al. cryptic risks to forest biosecurity associated with the global movement of commercial seed. Forests 2019; 10:459 [View Article]
    [Google Scholar]
  5. Franić I, Eschen R, Allan E, Hartmann M, Schneider S et al. Drivers of richness and community composition of fungal endophytes of tree seeds. FEMS Microbiol Ecol 2020; 96:1–10 [View Article] [PubMed]
    [Google Scholar]
  6. Abdollahzadeh J, Groenewald JZ, Coetzee M, Wingfield MJ, Crous PW. Evolution of lifestyles in Capnodiales. Studies in Mycology 2020; 95:381–414 [View Article]
    [Google Scholar]
  7. David JC. A contribution to the systematics of Cladosporium: revision of the fungi previously referred to Heterosporium. Mycol Papers 1997; 172:1–157
    [Google Scholar]
  8. Schubert K, Groenewald JZ, Braun U, Dijksterhuis J, Starink M et al. Biodiversity in the Cladosporium herbarum complex (Davidiellaceae, Capnodiales), with standardisation of methods for Cladosporium taxonomy and diagnostics. Stud Mycol 2007; 58:105–156 [View Article] [PubMed]
    [Google Scholar]
  9. Hawksworth DL. Fungal genera in urgent need of taxonomic work. Microbiol Sci 1986; 3:58
    [Google Scholar]
  10. Bensch K, Groenewald JZ, Dijksterhuis J, Starink-Willemse M, Andersen B et al. Species and ecological diversity within the Cladosporium cladosporioides complex (Davidiellaceae, Capnodiales). Stud Mycol 2010; 67:1–94 [View Article] [PubMed]
    [Google Scholar]
  11. Dugan FM, Braun U, Groenewald JZ, Crous PW. Morphological plasticity in Cladosporium sphaerospermum. Persoonia 2008; 21:9–16 [View Article] [PubMed]
    [Google Scholar]
  12. Wawrosch C, Malla PR, Kopp B. Micropropagation of Allium wallichii kunth, a threatened medicinal plant of Nepal. In Vitro CellDevBiol-Plant 2001; 37:555–557 [View Article]
    [Google Scholar]
  13. Liao ZY, Dang CL. Study on the ecotypes of Allium wallichii in jizu mountain, binchuan county, yunnan province. Acta Bot Yunnanica 1998; 20:439–444
    [Google Scholar]
  14. R. Lü R. Analysis of the composition and dominant species of seed-associated fungal communities and their effects on germination and pathogenicity. (Master’s thesis, Yunnan University); 2022
    [Google Scholar]
  15. Liu SH, Lu JP, Zhu RL, Dai FM. A rapid and simple extraction method for plant pathogenic fungi. Acta Phytopathol Sinica 2005; 4:362–365
    [Google Scholar]
  16. White TJ, Bruns TD, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications vol 18 1990 pp 315–322 [View Article]
    [Google Scholar]
  17. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999; 91:553–556 [View Article]
    [Google Scholar]
  18. O’Donnell K, Kistler HC, Cigelnik E, Ploetz RC. Multiple evolutionary origins of the fungus causing panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 1998; 95:2044–2049 [View Article] [PubMed]
    [Google Scholar]
  19. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article] [PubMed]
    [Google Scholar]
  20. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  21. Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 2015; 32:268–274 [View Article] [PubMed]
    [Google Scholar]
  22. Huelsenbeck JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 2001; 17:754–755 [View Article] [PubMed]
    [Google Scholar]
  23. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
    [Google Scholar]
  24. Rambaut A. FigTree v1.4.2; 2012 http://tree.bio.ed.ac.uk/
  25. Yan K, Pei Z, Meng L, Zheng Y, Wang L et al. Determination of community structure and diversity of seed-vectored endophytic fungi in Alpinia zerumbet. Front Microbiol 2022; 13:814864 [View Article] [PubMed]
    [Google Scholar]
  26. Qin Y, Pan X, Yuan Z. Seed endophytic microbiota in a coastal plant and phytobeneficial properties of the fungus Cladosporium cladosporioides. Fungal Ecology 2016; 24:53–60 [View Article]
    [Google Scholar]
  27. Zhong LY, Niu B, Xiang DB, Wu Q, Peng LX et al. Endophytic fungi in buckwheat seeds: exploring links with flavonoid accumulation. Front Microbiol 2024; 15:1353763 [View Article]
    [Google Scholar]
  28. Kim J, Roy M, Ahn S-H, Shanmugam G, Yang JS et al. Culturable endophytes associated with soybean seeds and their potential for suppressing seed-borne pathogens. Plant Pathol J 2022; 38:313–322 [View Article] [PubMed]
    [Google Scholar]
  29. Tobias TB, Farrer EC, Rosales A, Sinsabaugh RL, Suding KN et al. Seed-associated fungi in the alpine tundra: both mutualists and pathogens could impact plant recruitment. Fungal Ecol 2017; 30:10–18 [View Article]
    [Google Scholar]
  30. Yang N, Zhang WB, Wang D, Cao DD, Cao YY et al. A novel endophytic fungus strain of Cladosporium: its identification, genomic analysis, and effects on plant growth. Front Microbiol 2023; 14:1287582 [View Article]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.006730
Loading
/content/journal/ijsem/10.1099/ijsem.0.006730
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error