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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 3

sp. nov. and sp. nov., isolated from waste landfill No Access

Abstract

Two Gram-stain-positive, oxidase-negative, non-motile and rod-shaped strains (ASV49 and ASV81) were isolated from a waste landfill in Shanghai, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains are associated with members of the genus . Strains ASV49 and ASV81 were most closely related to JCM 31396 and CCTCC AA 2018025 with 98.5 and 98.0% 16S rRNA gene sequence similarities, respectively. The genomic DNA G+C contents of strains ASV49 and ASV81 were 69.5 and 70.5 mol %, respectively. Strains ASV49 and ASV81 exhibited the highest average nucleotide identity values of 81.7 and 85.3% against their closest relatives JCM 13591 and JCM 31396, respectively. Corresponding digital DNA–DNA hybridization values were 25.1 and 30.9%, respectively. Strains ASV49 and ASV81 grew optimally at pH 7.0 and 28 °C on Reasoner’s 2A. Strain ASV81 produced capsules, but ASV49 did not. Both isolates shared the following chemotaxonomic characteristics in common: the major fatty acids of anteiso-C, iso-C and anteiso-C; the respiratory quinones of MK-11, MK-12, MK-13 and MK-10; the polar lipids of diphosphatidylglycerol and phosphatidylglycerol; and the cell wall amino acids of ornithine, alanine, glycine and glutamic acid. Based on the genomic, phenotypic and chemical characteristics, strains ASV49 and ASV81 represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains are ASV49 (=GDMCC 1.4109=JCM 36480) and ASV81 (=GDMCC 1.4110=JCM 36481), respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , Microbacterium and novel species
Funding
This study was supported by the:
  • National Engineering Research Center of Solid-State Brewing (Award 19DZ2254400)
    • Principal Award Recipient: BingXie
© 2025 The Authors
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2025-12-13

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Microbacterium candidum sp. nov. and Microbacterium capsulatum sp. nov., isolated from waste landfill
Int J Syst Evol Microbiol 75, 006715 (2025); https://doi.org/10.1099/ijsem.0.006715
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