Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 3

sp. nov., a novel halophilic bacterium isolated from tidal flat sediment No Access

Abstract

A novel bacterial strain, MJW-29, was isolated from tidal flat sediment in Gochang, Republic of Korea. The isolate is Gram-stain-negative, facultatively anaerobic, gliding motile and short rods. The strain MJW-29 is oxidase and catalase positive. Growth is observed at 25–40 °C (optimum, 30 °C) and pH 5–9 (optimum, pH 7.0) in the presence of 0–9% (w/v) NaCl (optimum, 5.0% NaCl). The 16S rRNA gene sequence identity analysis showed that the strain MJW-29 is closely associated with KMU-156 (97.2%), AM1-D1 (97.2%), JBTF-M27 (97.0%) and KU5D5 (96.9%) from the family . The major respiratory quinone was Q-10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, phospholipid and an unidentified lipid. The major cellular fatty acids of type strain MJW-29 were C 7c/C 6c (64.5%). The genome length of the type strain MJW-29 is 4.9 Mbp (DNA G+C content, 62.1 mol%). It has 4638 coding genes and 43 tRNAs. The average identity values; average identity values; and biochemical, phylogenetic and physiological characteristics support the genotypic and phenotypic differentiation of strain MJW-29 from other members of the genus . The strain MJW-29 represents a novel species of the genus in the family , for which the name sp. nov. is proposed. The type strain is MJW-29 (=KCTC 8634=KEMB 20189=JCM 37145).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Genome Taxonomy Database Toolkit (GTDB-tk) , OrthoPhylo , phylogenetic analysis , taxonomy and tidal flat sediment
Funding
This study was supported by the:
  • KIMST the Sea Grant program (Award RS-2022-KS221563)
    • Principal Award Recipient: JaincyN Jayan
  • National Research Foundation (NRF) (Award 2022M3H9A1085063)
    • Principal Award Recipient: JaincyN Jayan
© 2025 The Authors

Erratum

A correction has been published for this content:
Corrigendum: gen. nov.,sp. nov., a novel halophilic bacterium isolated from tidal flat sediment
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.006705
2025-03-12
2026-02-15

Metrics

Loading full text...

Full text loading...

References

  1. Luo H, Moran MA. Evolutionary ecology of the marine Roseobacter clade. Microbiol Mol Biol Rev 2014; 78:573–587 [View Article] [PubMed]
     [Google Scholar]
  2. Buchan A, González JM, Moran MA. Overview of the marine Roseobacter lineage. Appl Environ Microbiol 2005; 71:5665–5677 [View Article] [PubMed]
     [Google Scholar]
  3. Parte AC, Sardà Carbasse J, Meier-Kolthoff JP, Reimer LC, Göker M. List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. Int J Syst Evol Microbiol 2020; 70:5607–5612 [View Article] [PubMed]
     [Google Scholar]
  4. Long C, Lu X-L, Gao Y, Jiao B-H, Liu X-Y. Description of a sulfitobacter strain and its extracellular cyclodipeptides. Evid Based Complement Alternat Med 2011; 2011:393752 [View Article] [PubMed]
     [Google Scholar]
  5. Beiralas R, Ozer N, Segev E. Abundant Sulfitobacter marine bacteria protect Emiliania huxleyi algae from pathogenic bacteria. ISME Commun 2023; 3:100 [View Article] [PubMed]
     [Google Scholar]
  6. Frank JA, Reich CI, Sharma S, Weisbaum JS, Wilson BA et al. Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 2008; 74:2461–2470 [View Article] [PubMed]
     [Google Scholar]
  7. Thompson JD, Gibson T, Higgins DG. Multiple sequence alignment using ClustalW and ClustalX. CP Bioinform 2003 [View Article]
     [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  9. Kannan L, Wheeler WC. Maximum parsimony on phylogenetic networks. Algorithms Mol Biol 2012; 7:9 [View Article] [PubMed]
     [Google Scholar]
  10. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article] [PubMed]
     [Google Scholar]
  11. Chaumeil P-A, Mussig AJ, Hugenholtz P, Parks DH. GTDB-Tk v2: memory friendly classification with the genome taxonomy database. Bioinformatics 2022; 38:5315–5316 [View Article] [PubMed]
     [Google Scholar]
  12. Yoon J. Isolation and polyphasic identification of Tateyamaria armeniaca sp. nov. FEMS Microbiol Lett 2019; 366:fnz182 [View Article] [PubMed]
     [Google Scholar]
  13. Yang Q, Ge Y-M, Iqbal NM, Yang X, Zhang X-L. Sulfitobacter alexandrii sp. nov., a new microalgae growth-promoting bacterium with exopolysaccharides bioflocculanting potential isolated from marine phycosphere. Antonie van Leeuwenhoek 2021; 114:1091–1106 [View Article] [PubMed]
     [Google Scholar]
  14. Park S, Chen S, Lee J-S, Kim W, Yoon J-H. Description of Sulfitobacter sediminilitoris sp. nov., isolated from a tidal flat. FEMS Microbiol Lett 2020; 367:fnaa074 [View Article] [PubMed]
     [Google Scholar]
  15. Park I, Cha I-T, Seo M-J. Thalassobius litorarius sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2016; 66:1666–1672 [View Article]
     [Google Scholar]
  16. Curry A, Appleton H, Dowsett B. Application of transmission electron microscopy to the clinical study of viral and bacterial infections: present and future. Micron 2006; 37:91–106 [View Article] [PubMed]
     [Google Scholar]
  17. Sass H, Köpke B, Rütters H, Feuerlein T, Dröge S et al. Tateyamaria pelophila sp. nov., a facultatively anaerobic alphaproteobacterium isolated from tidal-flat sediment, and emended descriptions of the genus Tateyamaria and of Tateyamaria omphalii. Int J Syst Evol Microbiol 2010; 60:1770–1777 [View Article] [PubMed]
     [Google Scholar]
  18. Palma V, Gutiérrez MS, Vargas O, Parthasarathy R, Navarrete P. Methods to evaluate bacterial motility and its role in bacterial-host interactions. Microorganisms 2022; 10:563 [View Article] [PubMed]
     [Google Scholar]
  19. Benson HJ. Microbiological Applications: A Laboratory Manual in General Microbiology, Complete Version, 8th Edn Boston Burr Ridge Dubuque: McGraw-Hill; 2002
     [Google Scholar]
  20. Smibert RM, Kreg NR. Phenotypic characterization. In Methods for General and Molecular Bacteriology American Society of Microbiology; 1994 pp 611–654
     [Google Scholar]
  21. Jayan JN, Kim H-S, Srinivasan S, Kim H-S, Yu J-Y et al. Gilvirhabdus luticola gen. nov., sp. nov., a mesophilic and halophilic bacterium isolated from tidal flat sediment. Int J Syst Evol Microbiol 2024; 74: [View Article]
     [Google Scholar]
  22. Han JH, Lee SE, Srinivasan S. Paenibacillus albiflavus sp. nov., a bacterium isolated from soil. Arch Microbiol 2021; 203:4973–4979 [View Article] [PubMed]
     [Google Scholar]
  23. Hameed A, Shahina M, Lai W-A, Lin S-Y, Liu Y-C et al. Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, Antarcticimonas flava Yang et al. 2009 and Hoppeia youngheungensis Kwon et al. 2014. Int J Syst Evol Microbiol 2015; 65:336–345 [View Article] [PubMed]
     [Google Scholar]
  24. Yoon J-H, Kang S-J, Lee M-H, Oh T-K. Description of Sulfitobacter donghicola sp. nov., isolated from seawater of the East Sea in Korea, transfer of Staleya guttiformis Labrenz et al. 2000 to the genus Sulfitobacter as Sulfitobacter guttiformis comb. nov. and emended description of the genus Sulfitobacter. Int J Syst Evol Microbiol 2007; 57:1788–1792 [View Article]
     [Google Scholar]
  25. Shiba T. Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain Bacteriochlorophyll a. Syst Appl Microbiol 1991; 14:140–145 [View Article]
     [Google Scholar]
  26. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for illumina sequence data. Bioinformatics 2014; 30:2114–2120 [View Article] [PubMed]
     [Google Scholar]
  27. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article] [PubMed]
     [Google Scholar]
  28. Lee I, Chalita M, Ha S-M, Na S-I, Yoon S-H et al. ContEst16S: an algorithm that identifies contaminated prokaryotic genomes using 16S RNA gene sequences. Int J Syst Evol Microbiol 2017; 67:2053–2057 [View Article] [PubMed]
     [Google Scholar]
  29. Kim D, Park S, Chun J. Introducing EzAAI: a pipeline for high throughput calculations of prokaryotic average amino acid identity. J Microbiol 2021; 59:476–480 [View Article] [PubMed]
     [Google Scholar]
  30. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T et al. The RAST server: rapid annotations using subsystems technology. BMC Genom 2008; 9:75 [View Article] [PubMed]
     [Google Scholar]
  31. Konstantinidis KT. Sequence-discrete species for prokaryotes and other microbes: a historical perspective and pending issues. mLife 2023; 2:341–349 [View Article] [PubMed]
     [Google Scholar]
  32. Buchan A, González JM, Chua MJ. Aerobic hydrocarbon-degrading Alphaproteobacteria: Rhodobacteraceae (Roseobacter). In McGenity TJ. eds Taxonomy, Genomics and Ecophysiology of Hydrocarbon-Degrading Microbes Cham: Springer International Publishing; pp 93–104 [View Article]
     [Google Scholar]
  33. Kurahashi M, Yokota A. Tateyamaria omphalii gen. nov., sp. nov., an alpha-proteobacterium isolated from a top shell Omphalius pfeifferi pfeifferi. Syst Appl Microbiol 2007; 30:371–375 [View Article] [PubMed]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.006705
Loading
Sulfitobacter sediminis sp. nov., a novel halophilic bacterium isolated from tidal flat sediment
Int J Syst Evol Microbiol 75, 006705 (2025); https://doi.org/10.1099/ijsem.0.006705
/content/journal/ijsem/10.1099/ijsem.0.006705
/content/journal/ijsem/10.1099/ijsem.0.006705
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error