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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 3

sp. nov., a DNA-hydrolysing bacterium isolated from intertidal sediment No Access

Abstract

A Gram-stain-negative, yellow-pigmented, non-flagellated, motile by gliding, aerobic and rod-shaped bacterial strain, designated SM2212, was isolated from intertidal sediment of Aoshan Bay in Qingdao, China. The strain grew at 5–35 °C and with 0.5–5.5% NaCl (w/v). It was able to reduce nitrate to nitrite and hydrolyse starch, gelatin and DNA. The phylogenetic trees based on the 16S rRNA genes and single-copy genes showed that strain SM2212 belonged to the genus within the family , sharing the highest 16S rRNA gene sequence similarity with the type strain of (97.1%) and 96.2–97.0% 16S rRNA gene sequence similarity with those of other known species in the genus. The major cellular fatty acids were summed feature 3 (C and/or C ), iso-C, iso-C3-OH, summed feature 9 (iso-C and/or 10-methyl C) and C. The major polar lipids were phosphatidylethanolamine and an unidentified lipid. The genomic DNA G+C content of strain SM2212 was 37.0 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between strain SM2212 and type strains of closely related known species were below 22.2 and 79.7%, respectively. Based on the polyphasic taxonomic analysis in this study, strain SM2212 is considered to represent a novel species in the genus , for which the name is proposed. The type strain is SM2212 (=KCTC 92980=MCCC 1K07684).

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Keyword(s): Christiangramia sediminicola sp. nov. , intertidal sediment and polyphasic taxonomy
Funding
This study was supported by the:
  • SKLMT Frontiers and Challenges Project (Award SKLMTFCP-2023-06)
    • Principal Award Recipient: Yu-ZhongZhang
  • National Science Foundation of China (Award 32371727)
    • Principal Award Recipient: Xiao-YanSong
  • National Science Foundation of China (Award 32471610)
    • Principal Award Recipient: Qi-LongQin
  • National Science Foundation of China (Award 42376106)
    • Principal Award Recipient: Ping-YiLi
  • National Science Foundation of China (Award 42076151)
    • Principal Award Recipient: ZhangXi-Ying
  • National Science Foundation of China (Award 32330001)
    • Principal Award Recipient: ZhangYu-Zhong
© 2025 The Authors
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2025-03-05
2026-04-14

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Christiangramia sediminicola sp. nov., a DNA-hydrolysing bacterium isolated from intertidal sediment
Int J Syst Evol Microbiol 75, 006703 (2025); https://doi.org/10.1099/ijsem.0.006703
/content/journal/ijsem/10.1099/ijsem.0.006703
/content/journal/ijsem/10.1099/ijsem.0.006703
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