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Strains P94T, B95T and R96T were isolated from apparently healthy fragments of the coral Montipora capitata, which were resistant to Vibrio coralliilyticus infection, from the reef surrounding Moku o Loʻe in Kāne‘ohe Bay, O‘ahu, Hawai‘i, USA, and were taxonomically evaluated using a polyphasic approach. Phylogenetic and phylogenomic analyses placed strains P94T, B95T and R96T within the Pseudoalteromonas genus and most closely related to Pseudoalteromonas luteoviolacea and Pseudoalteromonas rubra. Following genome sequencing of strains P94T, B95T and R96T, the average nt identity and in silico DNA–DNA hybridization comparisons with closely related strains resulted in values that fell below species-level cutoffs. Based on a polyphasic characterization and differences in genomic and taxonomic data, strains P94T, B95T and R96T represent novel species, for which the names Pseudoalteromonas ardens sp. nov., Pseudoalteromonas obscura sp. nov. and Pseudoalteromonas umbrosa sp. nov. are proposed. The type strains are R96T (=DSM 114998T=LMG 32870T), P94T (=DSM 114996T=LMG 32871T) and B95T (=DSM 114997T=LMG 32872T), respectively.
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