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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 2

sp. nov., isolated from surface seawater of the Weddell Sea in Antarctica No Access

Abstract

The poles of the Earth harbour many novel micro-organisms that have not yet been isolated and identified. Here, a Gram-stain-negative, motile, aerobic and rod-shaped bacterial strain, designated as wDCs-4, was isolated from surface seawater collected from the Weddell Sea in Antarctica. It grows at 4–40 °C (optimum 10–15 °C), pH 4–8 (optimum 7) and in the presence of 0–4% NaCl (w/v, optimum 0.5–1%). The complete 16S rRNA gene of strain wDCs-4 had maximum sequence identity with SM2-42 (97.2%), followed by MIL-1 (96.5%) and 197 (96.2%). Phylogenetic analyses based on the 16S rRNA gene and genome sequences showed that strain wDCs-4 was closely clustered with the members of the genus and formed an independent clade, which could be considered a monophyletic taxon. The average nucleotide identity values between strain wDCs-4 and the members of the genera and were 77.7–78.1 and 77.4–80.5%, respectively. The corresponding digital DNA‒DNA hybridization values are 19.5–20.1 and 20.5–22.4%, respectively. The major fatty acids (>5%) of strain wDCs-4 comprised summed feature 5 (C ante/C ,9 or C ,9/C ante) and C. The predominant respiratory was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, aminolipids and unknown polar lipids. The draft genome size was 4.58 Mbp, with a DNA G+C content of 53.4 mol%. On the basis of polyphasic analyses, strain wDCs-4 represented a novel species in the genus , for which the name sp. nov. was proposed. The type strain was wDCs-4 (=MCCC 1A20726=KCTC 8314).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Antarctica , Oceanobacter antarcticus sp. nov. , polyphasic taxonomy , surface seawater and Weddell Sea
Funding
This study was supported by the:
  • National Microbial Resource Center (Award NMRC-2024-9)
    • Principal Award Recipient: ZongzeShao
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principal Award Recipient: QiliangLai
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (Award 2019021)
    • Principal Award Recipient: ZongzeShao
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (Award 2020009)
    • Principal Award Recipient: ChunmingDong
  • National Key Research and Development Program (Award 2022YFC2807501)
    • Principal Award Recipient: ChunmingDong
© 2025 The Authors
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Oceanobacter antarcticus sp. nov., isolated from surface seawater of the Weddell Sea in Antarctica
Int J Syst Evol Microbiol 75, 006676 (2025); https://doi.org/10.1099/ijsem.0.006676
/content/journal/ijsem/10.1099/ijsem.0.006676
/content/journal/ijsem/10.1099/ijsem.0.006676
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