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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 2

sp. nov., sp. nov. and sp. nov.: three novel species of genus isolated from human breast milk No Access

Abstract

Three novel coccoid-shaped strains, designated 21WXBC0057M1, 21WXBC0044M1 and BJSWXB5TM5, were isolated from human breast milk in Wuxi, Jiangsu Province, China. These strains were facultative anaerobes, catalase-negative and Gram-positive. Through a comprehensive analysis of rRNA genes, protein-coding housekeeping genes and genomic phylogeny, we identified these strains as belonging to the genus . Specifically, strain 21WXBC0057M1 was phylogenetically most closely related to strain 21WXBC0044M1 was most closely related to and strain BJSWXB5TM5 displayed similarities to and . The pairwise average nucleotide identity and digital DNA–DNA hybridization values for these three strains were below 95 and 70%, respectively, indicating that they occupied evolutionary branches distinct from all previously validly published species. Distinctive phenotypic characteristics discriminated these novel species from the type strains of their most closely related species. The major cellular fatty acids in the three strains were C and C. Genome annotation and a thorough examination of carbohydrate-active enzyme distribution highlighted the observation that all strains possessed extensive capabilities for carbohydrate metabolism, particularly human milk oligosaccharides utilization. Thus, based on these findings, we proposed the classification of the strains as representing three novel species within the genus sp. nov. (type strain 21WXBC0057M1=GDMCC 1.4126=KCTC 25760), sp. nov. (type strain 21WXBC0044M1= GDMCC 1.4127=KCTC 25762) and sp. nov. (type strain BJSWXB5TM5=GDMCC 1.4130=KCTC 25759).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , gyrB , human breast milk , sodA , Streptococcus and taxonomy
Funding
This study was supported by the:
  • Fundamental Research Funds for the Central Universities
    • Principal Award Recipient: YangBo
  • Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province
    • Principal Award Recipient: ZhaoJianxin
  • National Key R&D Program of China (Award 2021YFD2100700)
    • Principal Award Recipient: YangBo
© 2025 The Authors
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2025-02-07
2026-02-16

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Streptococcus wuxiensis sp. nov., Streptococcus jiangnanensis sp. nov. and Streptococcus fermentans sp. nov.: three novel species of genus Streptococcus isolated from human breast milk
Int J Syst Evol Microbiol 75, 006674 (2025); https://doi.org/10.1099/ijsem.0.006674
/content/journal/ijsem/10.1099/ijsem.0.006674
/content/journal/ijsem/10.1099/ijsem.0.006674
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