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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 2

sp. nov., isolated from mangrove sediment and genome-based taxonomic analysis of the genus No Access

Abstract

A novel yellow-coloured bacterial strain MA10 was isolated from mangrove sediment and subjected to polyphasic taxonomic identification. Strain MA10 was Gram-negative, rod-shaped, catalase-positive and oxidase-positive. Carotenoid pigment was present, and flexirubin-type pigment was absent. The 16S rRNA gene of strain MA10 had the highest sequence similarity with HST1-43 of 94.5%. The genome size was 3.77 Mbp with a genomic G+C content of 36.3%. The phylogenetic analysis of the 16S rRNA gene sequence and whole-genome sequence showed that strain MA10 belonged to the genus of the family and tightly clustered with HST1-43. The digital DNA–DNA hybridization value and average nucleotide identity value between strain MA10 and HST1-43 were 20.4 and 76.7%, respectively. The major menaquinone was MK-6. The major fatty acids (>10 %) were iso-C (41.6%) and iso-C G (16.8%). The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids and three unidentified lipids. Based on the present polyphasic taxonomic study, strain MA10 was considered to represent a novel species of the genus , for which the name sp. nov. was proposed. The type strain was MA10 (=MCCC 1K09289=KCTC 102321). Additionally, the phylogeny of the 16S rRNA gene and whole-genome sequences found that the hitherto described nine species of the genus and presented polyphyletic clades, which could be divided into five different genera. The average amino acid identity value of 80% was chosen as the boundary of the five genera. Thus, except for the genus , four novel genera named gen. nov., gen. nov., gen. nov. and gen. nov. were proposed. This study provided valuable taxonomic analysis of the genus .

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Keyword(s): average amino acid identity , mangrove sediment , polyphasic taxonomy and Tamlana flava
Funding
This study was supported by the:
  • Quanzhou City Science and Technology Program (Award (2022C018R)
    • Principal Award Recipient: ZhaobinHuang
  • project of FuXiaQuan National Independent Innovation Demonstration Zone Collaborative Innovation Platform Project (Award 2023FX0001)
    • Principal Award Recipient: CongjieDai
© 2025 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006673
2025-02-07
2025-12-07

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/content/journal/ijsem/10.1099/ijsem.0.006673
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Tamlana flava sp. nov., isolated from mangrove sediment and genome-based taxonomic analysis of the genus Tamlana
Int J Syst Evol Microbiol 75, 006673 (2025); https://doi.org/10.1099/ijsem.0.006673
/content/journal/ijsem/10.1099/ijsem.0.006673
/content/journal/ijsem/10.1099/ijsem.0.006673
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