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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 2

sp. nov., a novel actinobacterium from mangrove sediment in Semarang city, Indonesia No Access

Abstract

A Gram-stain-positive, aerobic and non-motile actinobacterium, designated strain MR15.9, was isolated from sediment collected from a mangrove ecosystem in Semarang city, Indonesia. Strain MR15.9 grew at 4–37 °C (optimum 30 °C), pH 6.0–11.0 (optimum 7.0–8.0), with 0–10% (w/v) NaCl (optimum 0–3%). The genome of strain MR15.9 was 3.67 Mbp with 71.7 mol% G+C content. Phylogenetic analysis based on 16S rRNA gene sequence and genome sequence directed that strain MR15.9 formed a well-supported clade with CGMCC 1.15480 and 1P05MA and shared the highest similarity to CGMCC 1.15480 (98.9% sequence similarity) and 1P05MA (98.6% sequence similarity). However, the comparative genome analysis between strain MR15.9 and CGMCC 1.15480 gave average identity value of 85.2% and digital DNA–DNA hybridization value of 29.0%, which were below the statistical threshold for the delineation of the species. Chemotaxonomic data showed that the major fatty acids were anteiso-C, anteiso-C and iso-C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids. MK-8(H) and MK-9(H) were noted as the predominant respiratory quinones in this strain. The results of polyphasic characterization indicated that strain MR15.9 represents a novel species of the genus . The name sp. nov. is proposed, with the type strain MR15.9 (=MCCC 1K08875=KCTC 59105).

  • Received:
  • Accepted:
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Keyword(s): mangrove sediment , MR15.9T , polyphasic approach and Tersicoccus mangrovi
Funding
This study was supported by the:
  • Kunlun Talented People, High-end Innovation and Entrepreneurship Talents Plan of Qinghai Province
    • Principal Award Recipient: SunCheng-Hang
  • CAMS Innovation Fund for Medical Sciences (Award CIFMS 2021-I2M-1-028)
    • Principal Award Recipient: SunCheng-Hang
  • Foreign Youth Talent Program of the Ministry of Science and Technology of the People’s Republic of China (Award QN2022194001L)
    • Principal Award Recipient: SunCheng-Hang
© 2025 The Authors
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2025-02-03
2025-11-11

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Tersicoccus mangrovi sp. nov., a novel actinobacterium from mangrove sediment in Semarang city, Indonesia
Int J Syst Evol Microbiol 75, 006669 (2025); https://doi.org/10.1099/ijsem.0.006669
/content/journal/ijsem/10.1099/ijsem.0.006669
/content/journal/ijsem/10.1099/ijsem.0.006669
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