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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

sp. nov. and sp. nov., two novel species isolated from alkaline magnesite residues No Access

Abstract

Three bacterial strains, designated FZUC8N2.13, FBOR7N2.3 and FZUR7N2.5, were isolated from distinct magnesite residues in Spain. Phylogenetic and phylogenomic analysis places them within the genus . Strains FBOR7N2.3 and FZUR7N2.5 share 100% of similarity in the 16S rRNA gene sequence, and both are most closely related to AR-3-4 with which they share 97.5% of 16S rRNA gene similarity. Strain FZUC8N2.13 forms a distinct lineage most closely related to IMCC36792 with 97.7% 16S rRNA gene similarity. The closest phylogenomic neighbours of these three strains are DSM 1076, ‘’ BBQ-18 and PK15. The average nucleotide identity and digital DNA–DNA hybridization values between the three strains and closest members of the genus are below the threshold values of 95% and 70%, respectively. Strains FZUC8N2.13, FBOR7N2.3 and FZUR7N2.5 stain Gram-negative, are rod-shaped and form yellow colonies. Optimum growth occurs at 25 °C and pH 7. The genomic G+C contents are 33.4 mol% for strain FZUC8N2.13 and 33.2 mol% for strains FBOR7N2.3 and FZUR7N2.5. The major isoprenoid quinone is menaquinone 6. The major fatty acids are summed feature 3 (C ω7c and/or C ω6c) (22.6–31.1%), iso-C (13.6–16.2 %) and anteiso-C (8.7–10.5%). The polar lipids consist of two aminolipids, two aminophospholipids and one glycolipid. The phylogenetic, phylogenomic, phenotypic and chemotaxonomic data indicate that FZUC8N2.13, FBOR7N2.3 and FZUR7N2.5 are distinct from the described species of and should be classified as novel species, for which we propose the names for strain FZUC8N2.13 (=UCCCB 179=CECT 30977) and for strains FBOR7N2.3 (=UCCCB 178=CECT 30976) and FZUR7N2.5 (=UCCCB 216=CECT 31036).

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Keyword(s): alkaline residues , Flavobacterium magnesitis , Flavobacterium zubiriense and novel species
Funding
This study was supported by the:
  • Next Generation EU, Resilience and Recovery Plan (Award "AM2R", reference: 7253)
    • Principal Award Recipient: PaulaV. Morais
  • Horizon 2020 ERA-NET/ERA-MIN (Award ERA-MIN/0007/2019; Grant nº67)
    • Principal Award Recipient: PaulaV. Morais
  • Horizon 2020 (Award Grant nº821096)
    • Principal Award Recipient: PaulaV. Morais
  • Fundação para a Ciência e a Tecnologia (Award LA/P/0112/2020)
    • Principal Award Recipient: NotApplicable
  • Fundação para a Ciência e a Tecnologia (Award UID/EMS/00285/2020)
    • Principal Award Recipient: NotApplicable
© 2025 The Authors
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2026-02-11

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Flavobacterium magnesitis sp. nov. and Flavobacterium zubiriense sp. nov., two novel Flavobacterium species isolated from alkaline magnesite residues
Int J Syst Evol Microbiol 75, 006660 (2025); https://doi.org/10.1099/ijsem.0.006660
/content/journal/ijsem/10.1099/ijsem.0.006660
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