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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 2

sp. nov., a methanogen isolated from potential methane hydrate bearing area offshore southwestern Taiwan No Access

Abstract

A novel mesophilic, hydrogenotrophic methanogen, strain CWC-01, was isolated from the sediment sample of the piston core collected at station 3 at Good Weather Ridge area offshore southwestern Taiwan from ORI-902A cruise in 2009. Cells of strain CWC-01 are rod-shaped, measuring 0.4–0.5 µm in width and 1.0–2.0 µm in length. Strain CWC-01 utilizes formate and H/CO to produce methane, but not secondary alcohol, methanol, ethanol, methylamines or acetate as catabolic substrates. The optimal growth conditions are 37 °C, 0.043 M NaCl and pH 6.3. Growth effectors tests indicate tryptone, but not acetate and yeast extract, could stimulate the growth of strain CWC-01. Phylogenetic analysis of 16S rRNA gene reveals that strain CWC-01 is most closely related to Mic5c12, with 96.63% identity. Genome size of strain CWC-01 is 1.98 Mb and it is the smallest genome size of genus. The genomic DNA G+C content obtained from the genome sequence of strain CWC-01 is 44.15 mol%. Based on these phenotypic, phylogenetic and genomic analyses, we propose that strain CWC-01 represents a novel species in genus , for which the name sp. nov. is proposed. The type strain is CWC-01 (=BCRC AR10053=NBRC 113991).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): archaea , marine sediment , methane hydrate , Methanobacterium and methanogen
Funding
This study was supported by the:
  • Natural Science Foundation of Fujian Province (Award 2022H6035)
    • Principal Award Recipient: WangchuanXiao
  • Sanming University (Award 22YG13)
    • Principal Award Recipient: Chih-HungWu
  • Sanming University (Award 20YG09)
    • Principal Award Recipient: Sheng-ChungChen
  • Sanming University (Award 20YG04)
    • Principal Award Recipient: JingjingZhao
  • National Natural Science Foundation of China (Award 32101406)
    • Principal Award Recipient: YinLi
  • Department of Education, Fujian Province (Award JAT200646/B202037)
    • Principal Award Recipient: Chih-HungWu
  • Program for New Century Excellent Talents in University (Award KC180079)
    • Principal Award Recipient: Chih-HungWu
  • Natural Science Foundation of Fujian Province (Award 2021J011119, 2023J011018)
    • Principal Award Recipient: Chih-HungWu
  • National Science and Technology Council (Award NSTC 113-2320-B-039-016)
    • Principal Award Recipient: Shu-JungLai
  • Ministry of Science and Technology, Taiwan (Award MOST110-2320-B-039-058)
    • Principal Award Recipient: Shu-JungLai
  • Ministry of Science and Technology, Taiwan (Award MOST103/104/105/106/107-3113-M-005-001)
    • Principal Award Recipient: Mei-ChinLai
  • China Medical University, Taiwan (Award CMU110-N-31 and CMU113-MF-99)
    • Principal Award Recipient: Shu-JungLai
  • Central Geological Survey, Ministry of Economic Affairs (Award 99-5226904000-04-03)
    • Principal Award Recipient: Mei-ChinLai
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Methanobacterium aridiramus sp. nov., a methanogen isolated from potential methane hydrate bearing area offshore southwestern Taiwan
Int J Syst Evol Microbiol 75, 006658 (2025); https://doi.org/10.1099/ijsem.0.006658
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