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Abstract

Ciliates of the class Nassophorea Small & Lynn, 1981, inhabit a wide range of aquatic environments and serve as a trophic link in microbial food webs. However, their diversity remains underestimated. In the present study, the morphology and molecular phylogeny of Deroux, 1994, an insufficiently known nassulid ciliate, were investigated using an integrative taxonomic approach. The studied population was collected from a freshwater pond in Qingdao, China. Details of the live morphology and ciliature of are documented for the first time, and an improved species diagnosis is provided. Phylogenetic analyses of the small subunit ribosomal RNA gene sequence revealed that clusters within the family Nassulidae de Fromentel, 1874. However, evolutionary interrelationships within the Nassulidae remain obscure owing to under-sampling, the lack of sequence data for most nominal species, and low nodal support or unstable gene tree topologies.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 42276094)
    • Principal Award Recipient: XiaozhongHu
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/content/journal/ijsem/10.1099/ijsem.0.006651
2025-02-05
2026-03-14

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References

  1. Lynn DH. The Ciliated Protozoa. Characterization, Classification, and Guide to the Literature, 3rd ed Dordrecht: Springer; 2008
    [Google Scholar]
  2. Song W, Warren A, Hu X. Free-Living Ciliates in Bohai and Yellow Sea, China Beijing: Science Press; 2009
    [Google Scholar]
  3. Hu X, Lin X, Song W. Ciliate Atlas: Species Found in the South China Sea Beijing: Science Press; 2019 [View Article]
    [Google Scholar]
  4. Li T, Zhang T, Liu M, Zhang Z, Zhang J et al. Findings on three endocommensal scuticociliates (Protista, Ciliophora) from freshwater mollusks, including their morphology and molecular phylogeny with descriptions of two new species. Mar Life Sci Technol 2024; 6:212–235 [View Article] [PubMed]
    [Google Scholar]
  5. Feng X, Méndez-Sánchez D, Zhuang W, Li R, Pomahač O et al. Morphology, morphogenesis, and molecular characterization of Castula specialis sp. nov. (Ciliophora, Armophorea, Metopida). J Eukaryot Microbiol 2024; 71:e13014 [View Article] [PubMed]
    [Google Scholar]
  6. Jiang L, Wang C, Liao L, Al-Farraj SA, Warren A et al. Re-evaluation of the systematic position of the order Prostomatida (Protista: Ciliophora), with the establishment of two new genera and two new species. Zool J Linn Soc 2024; 200:849–864 [View Article]
    [Google Scholar]
  7. Li R, Zhuang W, Feng X, Zhu X, Hu X. Morphology and molecular phylogeny of three Parasonderia species including a new species (Ciliophora, Plagiopylea). Eur J Protistol 2024; 94:126087 [View Article] [PubMed]
    [Google Scholar]
  8. Liao L, Jiang L, Hu X. Integrative data of a novel ciliate (Alveolata, Ciliophora) propose the establishment of Heterodeviata nantongensis nov. sp. BMC Microbiol 2024; 24:27 [View Article] [PubMed]
    [Google Scholar]
  9. Wang C, Jiang L, Liao L, Qu Z, Kumar S et al. Taxonomic and phylogenetic studies on two marine Cyrtophoria ciliates (Protista: Ciliophora: Phyllopharyngea) with the description of a new species. Syst Biodiv 2023; 21:2269150 [View Article]
    [Google Scholar]
  10. Chi Y, Tang D, Lei J, Wei F, Al-Farraj SA et al. Highly divergent morphology but a close molecular phylogenetic relationship between two little-known ciliate genera Actinobolina and Papillorhabdos (Protozoa: Ciliophora: Litostomatea) with description of two new species. J Eukaryot Microbiol 2024; 71:e13007 [View Article] [PubMed]
    [Google Scholar]
  11. Jiang L, Wang C, Al-Farraj SA, Hines HN, Hu X. Morphological and molecular examination of the ciliate family Lagynusidae (Protista, Ciliophora, Prostomatea) with descriptions of two new genera and two new species from China. Mar Life Sci Technol 2023; 5:178–195 [View Article] [PubMed]
    [Google Scholar]
  12. Song W, Dong J, Lu X, Al-Farraj SA, Song W et al. Morphological, ontogenetic, and molecular investigations of freshwater hypotrich ciliates from China revealed a new genus Heterodeviata gen. nov. (Protista: Ciliophora), and a novel limnetic population of Deviata multilineae. Zool J Linnean Soc 2023; 199:263–279 [View Article]
    [Google Scholar]
  13. Wu T, Cheng T, Cao X, Jiang Y, Al-Rasheid KAS et al. On four epibiotic peritrichous ciliates (Protozoa, Ciliophora) found in Lake Weishan Wetland: morphological and molecular data support the establishment of a new genus, Parapiosoma gen. nov., and two new species. Mar Life Sci Technol 2023; 5:337–358 [View Article] [PubMed]
    [Google Scholar]
  14. Zhang G, Li Y, Gong R, Qiao Y, Al-Farraj SA et al. Taxonomy and molecular phylogeny of pleurostomatid ciliates from China with a description of two new species. Protist 2023; 174:125975 [View Article] [PubMed]
    [Google Scholar]
  15. Schewiakoff W. The organization and systematics of the infusoria aspirotricha. MéM Acad Impér Sci St Pétersb 1896; 4:1–395
    [Google Scholar]
  16. Adl SM, Bass D, Lane CE, Lukeš J, Schoch CL et al. Revisions to the classification, nomenclature, and diversity of eukaryotes. J Eukaryot Microbiol 2019; 66:4–119 [View Article] [PubMed]
    [Google Scholar]
  17. Gong J, Stoeck T, Yi Z, Miao M, Zhang Q et al. Small subunit rRNA phylogenies show that the class Nassophorea is not monophyletic (phylum ciliophora). J Eukaryot Microbiol 2009; 56:339–347 [View Article] [PubMed]
    [Google Scholar]
  18. Kivimaki KL, Bowditch BM, Riordan GP, Lipscomb DL. Phylogeny and systematic position of Zosterodasys (Ciliophora, Synhymeniida): a combined analysis of ciliate relationships using morphological and molecular data. J Eukaryot Microbiol 2009; 56:323–338 [View Article] [PubMed]
    [Google Scholar]
  19. Pan B, Chen X, Hou L, Zhang Q, Qu Z et al. Comparative genomics analysis of ciliates provides insights on the evolutionary history within “Nassophorea–Synhymenia–Phyllopharyngea” assemblage. Front Microbiol 2019; 10:2819 [View Article] [PubMed]
    [Google Scholar]
  20. Zhang Q, Yi Z, Fan X, Warren A, Gong J et al. Further insights into the phylogeny of two ciliate classes Nassophorea and Prostomatea (Protista, Ciliophora). Mol Phylogenet Evol 2014; 70:162–170 [View Article] [PubMed]
    [Google Scholar]
  21. Fan X, Pan H, Li L, Jiang J, Al-Rasheid KAS et al. Phylogeny of the poorly known ciliates, Microthoracida, a systematically confused taxon (Ciliophora), with morphological reports of three species. J Eukaryot Microbiol 2014; 61:227–237 [View Article] [PubMed]
    [Google Scholar]
  22. Zhang X, Sun C, Gong Z, Ma R, Ni B et al. Ultrastructure of Apocolpodidium etoschense (Ciliophora) and its systematics enlightenment for the class Nassophorea. Protist 2022; 173:125867 [View Article] [PubMed]
    [Google Scholar]
  23. Foissner W, Adam H. Morphologie und infraciliatur von Parafurgasonia sorex (Penard, 1922) nov. gen. und Obertrumia georgiana (Dragesco, 1972) nov. gen. (Protozoa: Ciliophora). Zool Anz 1922; 207:
    [Google Scholar]
  24. Foissner W, Agatha S, Berger H. Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa), with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Denisia 2002; 5:1–1459
    [Google Scholar]
  25. Kahl A. Die infusorien (Ciliata) der oldesloer Salzwasserstellen. Arch Hydrobiol 1928; 19:50–123
    [Google Scholar]
  26. Kahl A. Urtiere oder Protozoa I: wimpertiere oder Ciliata (Infusoria) 2. Holotricha außer den im 1.Teil behandelten Prostomata. Tierwelt Dtl 1931; 21:181–398
    [Google Scholar]
  27. Canter HM, Heaney SI, Lund JWG. The ecological significance of grazing on planktonic populations of cyanobacteria by the ciliate Nassula. New Phytologist 1990; 114:247–263 [View Article]
    [Google Scholar]
  28. Peck RK. Feeding behavior in the ciliate Pseudomicrothorax dubius is a series of morphologically and physiologically distinct events. J Protozool 1985; 32:492–501 [View Article]
    [Google Scholar]
  29. Tucker JB. Endocytosis and streaming of highly gelated cytoplasm alongside rows of arm-bearing microtubules in the ciliate Nassula. J Cell Sci 1978; 29:213–232 [View Article] [PubMed]
    [Google Scholar]
  30. Addicott JF. Predation and prey community structure: an experimental study of the effect of mosquito larvae on the protozoan communities of pitcher plants. Ecology 1974; 55:475–492 [View Article]
    [Google Scholar]
  31. Brabrand Å, Faafeng BA, Källqvist T, Nilssen JP. Biological control of undesirable cyanobacteria in culturally eutrophic lakes. Oecologia 1983; 60:1–5 [View Article] [PubMed]
    [Google Scholar]
  32. Clément-Iftode F. Sur une Sphaerophrya ectoparasite de Nassula elegans. Protistologica 1967; 3:127–132
    [Google Scholar]
  33. Fauré-Fremiet E Podophrya parasitica nov. sp. Bull Biol Fr Belg 1945; 79:85–97
    [Google Scholar]
  34. Fryd-Versavel G, Iftode F, Deroux G. La famille des Nassulidae et l’ancien genre Nassula. J Protozool 1980; 27:79A
    [Google Scholar]
  35. Deroux G. Sous-classe des Nassulia Jankowski, 1967. Traité Zool 1994; 2:585–600
    [Google Scholar]
  36. CORLISS JO. Silver impregnation of ciliated protozoa by the Chatton-Lwoff technic. Stain Technol 1953; 28:97–100 [View Article] [PubMed]
    [Google Scholar]
  37. Medlin L, Elwood HJ, Stickel S, Sogin ML. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 1988; 71:491–499 [View Article] [PubMed]
    [Google Scholar]
  38. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Ser 1999; 41:95–98
    [Google Scholar]
  39. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A et al. MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Syst Biol 2012; 61:539–542 [View Article] [PubMed]
    [Google Scholar]
  40. Nylander J. MrModeltest v2. Program distributed by the Author. In Evolutionary Biology Centre, Uppsala University 2004
    [Google Scholar]
  41. Gouy M, Guindon S, Gascuel O. SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 2010; 27:221–224 [View Article] [PubMed]
    [Google Scholar]
  42. Foissner W. Miscellanea nomenclatorica ciliatea (Protozoa: Ciliophora). Arch Protistenkd 1987; 133:219–235 [View Article]
    [Google Scholar]
  43. International Commission on Zoological Nomenclature (ICZN Intemational Code of Zoological Nomenclature London: International Trust for Zoological Nomenclature; 1999
    [Google Scholar]
  44. Foissner W, O’Donoghue P. Morphology and infraciliature of some freshwater ciliates (Protozoa : Ciliophora) from Western and South Australia. Invertebr Taxon 1989; 3:661 [View Article]
    [Google Scholar]
  45. Fauré-Fremiet E. The genus Cyclogramma Perty, 1852. J Protozool 1967; 14:456–464 [View Article] [PubMed]
    [Google Scholar]
  46. Liao W, Fan X, Zhang Q, Xu Y, Gu F. Morphology and phylogeny of two novel ciliates, Arcanisutura chongmingensis n. gen., n. sp. and Naxella paralucida n. sp. from Shanghai, China. J Eukaryot Microbiol 2018; 65:48–60 [View Article] [PubMed]
    [Google Scholar]
  47. Eisler K. Electron microscopical observations on the ciliate Furgasonia blochmanni Fauré-Fremiet, 1967: part I: an update on morphology. Eur J Protistol 1988; 24:75–93 [View Article] [PubMed]
    [Google Scholar]
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