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Abstract

Two Gram-stain-negative cocci anaerobes were isolated from pig faeces and designated as strains YH-vei2232 and YH-vei2233. Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates were most closely related to KCTC 5967, with 97.0% similarity. Analysis of housekeeping gene sequences () revealed the strain formed a sub-cluster within the genus . The average nucleotide identities between the two isolates and the most closely related strains within genus were <75.0%. The major fatty acids were Summed Feature 8 and C c. The cell wall peptidoglycan contained -diaminopimelic acid. The major metabolic end products of isolates were propionic and acetic acids. The genomic DNA G+C contents of both strains were 40.1 mol%. The chemotaxonomic, phenotypic and phylogenetic properties of YH-vei2232 (=KCTC 25748=NBRC 116427) and YH-vei2233 (=KCTC 25749=NBRC 116428) suggest that they represent a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2021R1A2C2009051)
    • Principal Award Recipient: Joong-KiKook
  • Korea Research Institute of Bioscience and Biotechnology (Award KGS1022423)
    • Principal Award Recipient: Young-HyoChang
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/content/journal/ijsem/10.1099/ijsem.0.006643
2025-01-15
2026-02-18

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References

  1. Parte AC. Lpsn - List of prokaryotic names with standing in nomenclature (bacterio.net), 20 years on. Int J Syst Evol Microbiol 2018; 68:1825–1829 [View Article] [PubMed]
    [Google Scholar]
  2. Carlier JP. Veillonella. In Bergey’s Manual of Systematics of Archaea and Bacteria 2015 pp 1–11 [View Article]
    [Google Scholar]
  3. Delwiche EA, Pestka JJ, Tortorello ML. The veillonellae: gram-negative cocci with a unique physiology. Annu Rev Microbiol 1985; 39:175–193 [View Article] [PubMed]
    [Google Scholar]
  4. Parte AC, Sardà Carbasse J, Meier-Kolthoff JP, Reimer LC, Göker M. List of prokaryotic names with standing in nomenclature (LPSN) moves to the DSMZ. Int J Syst Evol Microbiol 2020; 70:5607–5612 [View Article] [PubMed]
    [Google Scholar]
  5. Rogosa M. Anaerobic gram-negative cocci. Family I. Veillonellaceae rogosa 1971. In Krieg NR, Holt JG. eds Bergey’s Manual of Systematic Bacteriology vol 1 Baltimore: Williams & Wilkins; 1984 pp 680–685
    [Google Scholar]
  6. Kolenbrander P. The genus Veillonella. In Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E. eds In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd Edn vol 4 New York: Springer; 2006 pp 1022–1040
    [Google Scholar]
  7. Aujoulat F, Bouvet P, Jumas-Bilak E, Jean-Pierre H, Marchandin H. Veillonella seminalis sp. nov., a novel anaerobic gram-stain-negative coccus from human clinical samples, and emended description of the genus Veillonella. Int J Syst Evol Microbiol 2014; 64:3526–3531 [View Article] [PubMed]
    [Google Scholar]
  8. Mashima I, Theodorea CF, Djais AA, Kunihiro T, Kawamura Y et al. Veillonella nakazawae sp. nov., an anaerobic gram-negative coccus isolated from the oral cavity of Japanese children. Int J Syst Evol Microbiol 2021; 71: [View Article] [PubMed]
    [Google Scholar]
  9. Zhang SM, Huang SL. The commensal anaerobe Veillonella dispar reprograms its lactate metabolism and short-chain fatty acid production during the stationary phase. Microbiol Spectr 2023; 11:e0355822 [View Article] [PubMed]
    [Google Scholar]
  10. Arrieta M-C, Stiemsma LT, Dimitriu PA, Thorson L, Russell S et al. Early infancy microbial and metabolic alterations affect risk of childhood asthma. Sci Transl Med 2015; 7:307ra152 [View Article]
    [Google Scholar]
  11. Scheiman J, Luber JM, Chavkin TA, MacDonald T, Tung A et al. Meta-omics analysis of elite athletes identifies a performance-enhancing microbe that functions via lactate metabolism. Nat Med 2019; 25:1104–1109 [View Article] [PubMed]
    [Google Scholar]
  12. Wei Y, Li Y, Yan L, Sun C, Miao Q et al. Alterations of gut microbiome in autoimmune hepatitis. Gut 2020; 69:569–577 [View Article] [PubMed]
    [Google Scholar]
  13. Mashima I, Kamaguchi A, Miyakawa H, Nakazawa F. Veillonella tobetsuensis sp. nov., an anaerobic, gram-negative coccus isolated from human tongue biofilms. Int J Syst Evol Microbiol 2013; 63:1443–1449 [View Article] [PubMed]
    [Google Scholar]
  14. Djais AA, Theodorea CF, Mashima I, Otomo M, Saitoh M et al. Identification and phylogenetic analysis of oral Veillonella species isolated from the saliva of Japanese children. F1000Res 2019; 8:616 [View Article] [PubMed]
    [Google Scholar]
  15. Theodorea CF, Djais AA, Mashima I, Otomo M, Saitoh M et al. Phylogenetic study of oral Veillonella isolates from saliva of children in hokkaido. Dent J Heal Sci Univ Hokkaido 2019; 38:37–46
    [Google Scholar]
  16. Bai L, Paek J, Shin Y, Kim H, Kim SH et al. Aerococcus kribbianus sp. nov., a facultatively anaerobic bacterium isolated from pig faeces. Int J Syst Evol Microbiol 2024; 74: [View Article] [PubMed]
    [Google Scholar]
  17. Bai L, Paek J, Shin Y, Kim H, Kim SH et al. Lacticaseibacillus parakribbianus sp. nov., isolated from a pig farm faeces dump. Int J Syst Evol Microbiol 2023; 73: [View Article] [PubMed]
    [Google Scholar]
  18. Chun J, Goodfellow M. A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 1995; 45:240–245 [View Article] [PubMed]
    [Google Scholar]
  19. Paek J, Bai L, Shin Y, Kim H, Kook JK et al. Description of Paenibacillus dokdonensis sp. nov., a new bacterium isolated from soil. Int J Syst Evol Microbiol 2019; 71: [View Article] [PubMed]
    [Google Scholar]
  20. ROGOSA M. A selective medium for the isolation and enumeration of the Veillonella from the oral cavity. J Bacteriol 1956; 72:533–536 [View Article] [PubMed]
    [Google Scholar]
  21. Paek J, Shin Y, Kim J-S, Kim H, Kook J-K et al. Description of Absiella argi gen. nov., sp. nov., and transfer of Eubacterium dolichum and Eubacterium tortuosum to the genus Absiella as Absiella dolichum comb. nov. and Absiella tortuosum comb. nov. Anaerobe 2017; 48:70–75 [View Article] [PubMed]
    [Google Scholar]
  22. Schumann P. Peptidoglycan structure. Methods Microbiol 2011; 38:101–129 [View Article]
    [Google Scholar]
  23. Ma S, Huang Y, Wang C, Fan H, Dai L et al. Defluviitalea raffinosedens sp. nov., a thermophilic, anaerobic, saccharolytic bacterium isolated from an anaerobic batch digester treating animal manure and rice straw. Int J Syst Evol Microbiol 2017; 67:1607–1612 [View Article] [PubMed]
    [Google Scholar]
  24. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article] [PubMed]
    [Google Scholar]
  25. Rodriguez-R LM, Konstantinidis KT. The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes. PeerJ Preprintse1900v1 2016; 4 [View Article]
    [Google Scholar]
  26. Meier-Kolthoff JP, Carbasse JS, Peinado-Olarte RL, Göker M. TYGS and LPSN: a database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes. Nucleic Acid Res 2022; 50:D801–D807 [View Article]
    [Google Scholar]
  27. Alanjary M, Steinke K, Ziemert N. AutoMLST: an automated web server for generating multi-locus species trees highlighting natural product potential. Nucleic Acid Res 2019; 47:W276–W282 [View Article] [PubMed]
    [Google Scholar]
  28. Riesco R, Trujillo ME. Update on the proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2024; 74:006300 [View Article] [PubMed]
    [Google Scholar]
  29. Rodriguez-R LM, Conrad RE, Viver T, Feistel DJ, Lindner BG et al. An ANI gap within bacterial species that advances the definitions of intra-species units. mBio 2024; 15:e0269623 [View Article] [PubMed]
    [Google Scholar]
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