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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

sp. nov., a crude oil aggregation-forming anaerobic bacterium isolated from marine sediment No Access

Abstract

https://doi.org/10.1099/ijsem.0.006671

A crude oil aggregation-forming, strictly anaerobic, Gram-stain-positive, spore-forming, rod-shaped, motile and mesophilic bacterium, named strain SH18-2, was isolated from marine sediment near Sado Island in the Sea of Japan. The temperature, salinity and pH ranges of this strain for the growth were 15–40 °C (optimum 35 °C), 0.5–6.0% NaCl (w/v; optimum 2.0%) and pH 6.0–8.0 (optimum 7.5), respectively. A phylogenetic analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus . It was most closely related to A-1 (97.02% homology). The draft genome size was 5616089 bp, with a G+C content of 29.7 mol%. The digital DNA–DNA hybridization values determined using the Genome-to-Genome Distance Calculator and the average nucleotide identity between this strain and A-1 were 20.30 and 78.24%, respectively. Whole-organism hydrolysates of strain SH18-2 contained diaminopimelic acid; the major fatty acid was C and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, glycolipid and three unidentified polar lipids. From the results of genotypic, phenotypic and chemotaxonomic analyses, SH18-2 (=NBRC 116030=DSM 115762) is the type strain and represents a novel species of the genus , and the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): aggregation , anaerobic bacteria , Clostridium , crude oil and marine sediment
Funding
This study was supported by the:
  • ENEOS Corporation
    • Principal Award Recipient: TakeshiKobayashi
© 2025 The Authors

Erratum

A correction has been published for this content:
Erratum: sp. nov., a crude oil aggregation-forming anaerobic bacterium isolated from marine sediment
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2025-01-15
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Clostridium sediminicola sp. nov., a crude oil aggregation-forming anaerobic bacterium isolated from marine sediment
Int J Syst Evol Microbiol 75, 006641 (2025); https://doi.org/10.1099/ijsem.0.006641
/content/journal/ijsem/10.1099/ijsem.0.006641
/content/journal/ijsem/10.1099/ijsem.0.006641
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