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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

sp. nov., sp. nov. and sp. nov. isolated from the phyllosphere of litchi No Access

Abstract

Three aerobic, pink-pigmented, Gram-negative, motile and rod-shaped bacterial strains, designated SD21, SI9 and SB2, were isolated from the phyllosphere of healthy litchis collected from three main producing sites of Guangdong Province, PR China. The 16S rRNA gene analysis showed that strains SD21 and SI9 belonged to the genus (.) with the highest similarity to DSM 19563 (98.7%) and CBMB27 (99.8%), respectively, while strain SB2 belonged to the genus (.) and showed the highest similarity to DSM 14458 (98.6%). Phylogenomic analysis based on 92 core genes clearly showed that the most closely related type strains of SD21, SI9 and SB2 were DSM 19563, ICMP 17619 and CGMCC 1.6474, respectively. The ANI and dDDH values between the three isolates and their most closely related type strains were 85.6‒90.1% and 29.5‒40.4%, respectively, much below the threshold values for species delimitation. The isolates showed clear differences from their closely related type strains in terms of growth conditions, enzyme activities, substrates assimilation and contents of the major fatty acids. They all took summed feature 8 (C ω7c and/or C ω6c) as the major fatty acid, ubiquinone 10 as the predominant respiratory quinone and phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine as the major polar lipids. The phenotypic, phylogenetic and chemotaxonomic analyses with genome comparison strongly support that the isolates represent three distinct novel species within the genera of and , for which the names sp. nov., sp. nov. and sp. nov. are proposed, with SD21 (=GDMCC 1.4327=KCTC 8300), SI9 (=GDMCC 1.4329=KCTC 8298) and SB2 (=GDMCC 1.4328=KCTC 8299) as the type strains, respectively.

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  • Accepted:
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Keyword(s): litchi , Methylobacterium , Methylorubrum , methylotrophic bacteria and phyllosphere
Funding
This study was supported by the:
  • Special Fund Project for Science and Technology Innovation Strategy of Guangdong Province (Award 2019QN01N107)
    • Principle Award Recipient: XinqiangXie
  • the Guangdong Strategic Special Fund for Rural Revitalization (Award 2023-WBH-00-001)
    • Principle Award Recipient: HonghuiZhu
  • the GDAS’ Project of Science and Technology Development (Award 2022GDASZH-2022010201)
    • Principle Award Recipient: HonghuiZhu
  • the Guangdong Special Support Program (Award 2021JC06N628)
    • Principle Award Recipient: HonghuiZhu
  • the Science and Technology Program of Guangdong Province (Award 2021B1212050022)
    • Principle Award Recipient: HonghuiZhu
© 2025 The Authors
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2025-01-09
2025-01-25
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Methylobacterium litchii sp. nov., Methylobacterium guangdongense sp. nov. and Methylorubrum subtropicum sp. nov., isolated from the phyllosphere of litchi
Int J Syst Evol Microbiol 75, 006639 (2025); https://doi.org/10.1099/ijsem.0.006639
/content/journal/ijsem/10.1099/ijsem.0.006639
/content/journal/ijsem/10.1099/ijsem.0.006639
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