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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

sp. nov., a bacterium isolated from the roots of the aquatic plant No Access

Abstract

A polyphasic taxonomic study was carried out on strain T5W1, isolated from the roots of the aquatic plant . This isolate is Gram-negative, rod-shaped, motile, aerobic and non-pigmented. Nearly complete 16S rRNA gene sequence homology related the strain to , with 98.4% similarity to , and . Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) with the closest phylogenetic neighbour of T5W1 showed differences at the species level, further confirmed by differences in several physiological characteristics. The main fatty acids are summed feature 3 (C and/or C ), C , and C. The DNA G+C content is 59.3 mol%. Q-9 is the primary ubiquinone found, and phosphatidylethanolamine is the dominant polar lipid, with lesser amounts of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylserine. Based on the results obtained, this bacterium is assigned to the genus as a new species with the name sp. nov., type strain T5W1 (=NRRL B-65714 =DSM 118085).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): microbe–plant interactions , plant growth-promoting rhizobacteria , Pseudomonas , Pseudomonas spirodelae , root-associated bacteria and Spirodella polyrhiza
Funding
This study was supported by the:
  • Department of Biology, Slippery Rock University
    • Principle Award Recipient: ChrisMaltman
  • Slippery Rock University Faculty Student Research Grant
    • Principle Award Recipient: ChrisMaltman
  • Commonwealth of Pennsylvania University Biologists
    • Principle Award Recipient: KarlieVandeborne
© 2025 The Authors
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2025-01-25
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Pseudomonas spirodelae, sp. nov., a bacterium isolated from the roots of the aquatic plant Spirodela polyrhiza
Int J Syst Evol Microbiol 75, 006637 (2025); https://doi.org/10.1099/ijsem.0.006637
/content/journal/ijsem/10.1099/ijsem.0.006637
/content/journal/ijsem/10.1099/ijsem.0.006637
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