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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

sp. nov., isolated from a patient with ruptured appendicitis Open Access

Abstract

A clinical isolate, R131, was isolated from the peritoneal swab of a patient who suffered from ruptured appendicitis with abscess and gangrene in Hong Kong in 2018. Cells are facultatively anaerobic, non-motile, Gram-positive coccobacilli. Colonies were small, grey, semi-translucent, low convex and alpha-haemolytic. The bacterium grew on blood agar but not on Brain Heart Infusion (BHI) and Mueller–Hinton agars. It was negative for catalase, oxidase, indole and aesculin hydrolysis. The initial identification attempts via matrix-assisted laser desorption ionization–time of flight mass spectrometry and 16S rRNA gene sequencing yielded inconclusive results. The 16S rRNA gene analysis showed that R131 shared >99% nucleotide identity with certain uncultured bacteria. In this retrospective investigation, a complete genome of R131 was constructed, disclosing a DNA G+C content of 64%. Phylogenetic analysis showed that the bacterium was mostly related to WB03_NA08, which was first described in 2020. However, its 16S rRNA gene shared only 94.15% nucleotide identity with that of WB03_NA08. Notably, the orthoANI between R131 and WB03_NA08 was 67.81%. A pan-genome analysis encompassing R131 and 4 genomes showed 986 core gene clusters shared with the species, along with 601 cloud genes. The average nucleotide identity comparisons within the pan-genome analysis ranged from 59.78 to 62.51% between R131 and the other species. Correspondingly, the dDDH values ranged from 19.20 to 22.30%, while the POCP values spanned from 57.48 to 60.94%. Therefore, a novel species, sp. nov., is proposed. The type strain is R131 (=JCM 36615=LMG 33627).

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Keyword(s): peritoneal , ruptured appendicitis and Scrofimicrobium appendicitidis sp. nov.
Funding
This study was supported by the:
  • Health and Medical Research Fund (Award 21200092)
    • Principal Award Recipient: GilmanKit-Hang Siu
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2025-01-21
2026-02-15

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Scrofimicrobium appendicitidis sp. nov., isolated from a patient with ruptured appendicitis
Int J Syst Evol Microbiol 75, 006633 (2025); https://doi.org/10.1099/ijsem.0.006633
/content/journal/ijsem/10.1099/ijsem.0.006633
/content/journal/ijsem/10.1099/ijsem.0.006633
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