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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

sp. nov. isolated from a human blood culture No Access

Abstract

A novel species, strain BD556, isolated from blood, was identified at Mayo Clinic, Rochester, MN, USA. After failing definitive identification using MALDI-ToF MS and partial 16S rRNA gene sequencing, BD556 was characterized using a polyphasic approach, including phenotypic, biochemical and whole-genome sequencing methods. BD556 was a Gram-positive rod with clubbed ends, facultatively anaerobic, catalase-positive, oxidase-negative and non-motile. Colonies were white, opaque and non-haemolytic with halo-like edges. BD556 grew at 35 °C in room air, with CO and under anaerobic conditions. BD556 grew well in 0 and 6% NaCl and weakly in 10% NaCl. The genome size was 2 349 779 bp with a G+C content of 60.39%. Phylogenetic analysis using 16S rRNA gene sequence analysis, average nucleotide identity and digital DNA–DNA hybridization between the genome of BD556 and the closest type strains from the Type Strain Genome Server database yielded separation values well beyond those required for species delineation. Chemotaxonomic analyses of BD556 revealed ribose, arabinose and galactose as whole-cell sugars and an A1γ -diaminopimelic acid-direct peptidoglycan type. The major cellular fatty acids were C (21.0%), C (14.8%), C ω9c (26.2%), C (13.3%) and C ω9c (18.3%). Polar lipids included diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids and phospholipids. BD556 also contained mycolic acids (32–36 carbons) typical of corynebacteria. The respiratory quinones were dominated by MK-8(H) (71.2%) and MK-9(H) (25.9%), with smaller amounts of MK-7(H) and MK-10(H). The results presented support the tenet that BD556 (=TSD 427=NCTC 15078) is a novel species for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Corynebacterium , novel species and whole-genome sequence
Funding
This study was supported by the:
  • Office of Public Health Preparedness and Response (Award CK22-2204)
    • Principal Award Recipient: ApplicableNot
  • Swiss National Science Foundation (Award P500PM_214215)
    • Principal Award Recipient: C HerrenSebastian
© 2025 https://www.mayoclinic.org/copyright/
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2025-01-31
2026-04-19

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Corynebacterium mayonis sp. nov. isolated from a human blood culture
Int J Syst Evol Microbiol 75, 006632 (2025); https://doi.org/10.1099/ijsem.0.006632
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