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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

Four novel nontuberculous mycobacteria species: sp. nov., sp. nov., sp. nov. and sp. nov. revived from a historical Danish strain collection Open Access

Abstract

Four novel nontuberculous mycobacteria were discovered from a historical strain collection at the International Reference Laboratory of Mycobacteriology at Statens Serum Institut in Copenhagen, Denmark. Phylogenetic analysis combining the 16S , internal transcribed spacer and 23S elements, as well as a single-copy core-gene (, , and ) analysis of these freeze-dried mycobacteria, clinically isolated from gastric lavage samples between 1948 and 1955, showed to be associated with type strains grouping within the Terra and Fortuitum-Vaccae clade. Phenotypic characteristics, biochemical properties and fatty acid and mycolic acid profiles supported the classification as novel strains. A genomic comparison to the closest related type strain was done by calculating average nucleotide identity and DNA:DNA hybridization values, which showed 87.9% and 33.0% for Mu0050, 85.2% and 27.4% for Mu0053, 85.3% and 27.6% for Mu0083 and 93.3% and 50.1% for Mu0102, respectively. The names proposed for the new species are sp. nov. (Mu0050=ITM 501390=CCUG 77525), sp. nov. (Mu0053=ITM 501391=CCUG 77526), sp. nov. (Mu0083=ITM 501392=CCUG 77527) and sp. nov. (Mu0102=ITM 501393=CCUG 77528).

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Keyword(s): Mycobacterium burgundiense sp. nov. , Mycobacterium holstebronense sp. nov. , Mycobacterium kokjensenii sp. nov. , Mycobacterium wendilense sp. nov. , nontuberculous mycobacteria , novel species and taxonomic description
Funding
This study was supported by the:
  • Danmarks Frie Forskningsfond (Award 3127-00035B)
    • Principle Award Recipient: Emilie Sinding IversenXenia
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2025-01-07
2025-01-15
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Four novel nontuberculous mycobacteria species: Mycobacterium wendilense sp. nov., Mycobacterium burgundiense sp. nov., Mycobacterium kokjensenii sp. nov. and Mycobacterium holstebronense sp. nov. revived from a historical Danish strain collection
Int J Syst Evol Microbiol 75, 006620 (2025); https://doi.org/10.1099/ijsem.0.006620
/content/journal/ijsem/10.1099/ijsem.0.006620
/content/journal/ijsem/10.1099/ijsem.0.006620
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