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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 12

sp. nov. isolated from watermelon and muskmelon in Serbia Open Access

Abstract

Six strains isolated from muskmelon and watermelon seedlings affected by stem rot and wilting in Serbia were reported as based on pathogenicity, LOPAT and cell wall fatty acid analyses. Recent bacterial isolates from cucurbit crops displaying -like symptoms in Alabama, USA, were identified as , prompting polyphasic re-evaluation of the Serbian strains. All six strains were found to cause severe disease in watermelon and squash seedlings under greenhouse conditions. Strains KFB 138 and KFB 140 underwent whole-genome sequencing and were found to have the highest level of 16S rRNA similarity to LJ2 (both 99.87%). Phylogenies based on housekeeping genes and core-genome analysis placed both strains into phylogroup 11 of the species complex, with KFB 138 forming a lineage basal to all other phylogroup 11 members. In core-genome phylogeny, KFB 140 was placed into a clade alongside LJ2. Average nucleotide identity based on (ANIb) identified KFB 140 as a member of (95.85%), though KFB 138 did not produce an ANIb value over 95% to any type strain to which it was compared. Values for DNA–DNA hybridization for both strains were below 70% to all reference strains tested, though KFB 140 was found to be most similar to (68.2%). KFB 138 and KFB 140 were further characterized using the online Type Genome Server, biochemical profiling with the Biolog Gen III MicroPlate system, matrix-assisted laser desorption/ionization time of flight mass spectrometry and imaged with transmission electron microscopy. From the results of the above analyses, we conclude that KFB 140 is a member of the species and that KFB 138 represents a novel species, for which we propose the name (KFB 138, NCPPB 4762=LMG 33366), named for its location of isolation.

  • Received:
  • Accepted:
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Keyword(s): bacteria , cucurbits , Pseudomonas and watermelon
Funding
This study was supported by the:
  • National Institute of Food and Agriculture (Award 2019-51181-30019)
    • Principal Award Recipient: NotApplicable
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-12-19
2026-01-18

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References

  1. Lalucat J, Mulet M, Gomila M, García-Valdés E. Genomics in bacterial taxonomy: impact on the genus Pseudomonas. Genes 2020; 11:139 Epub ahead of print 1 February 2020 [View Article] [PubMed]
     [Google Scholar]
  2. Parte AC, Sardà Carbasse J, Meier-Kolthoff JP, Reimer LC, Göker M. List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. Int J Syst Evol Microbiol 2020; 70:5607–5612 [View Article] [PubMed]
     [Google Scholar]
  3. Peix A, Ramírez-Bahena MH, Velázquez E. The current status on the taxonomy of Pseudomonas revisited: an update. Infect Genet Evol 2018; 57:106–116 [View Article] [PubMed]
     [Google Scholar]
  4. Spiers AJ, Buckling A, Rainey PB. The causes of Pseudomonas diversity. Microbiology 2000; 146:2345–2350 [View Article]
     [Google Scholar]
  5. Morris CE, Sands DC, Vinatzer BA, Glaux C, Guilbaud C et al. The life history of the plant pathogen Pseudomonas syringae is linked to the water cycle. ISME J 2008; 2:321–334 [View Article] [PubMed]
     [Google Scholar]
  6. Bergan T. Human and animal-pathogenic members of the genus Pseudomonas. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG. eds The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria Berlin: Springer; 1981 pp 666–700 [View Article]
     [Google Scholar]
  7. Schroth MN, Hildebrand DC, Panopoulos N. Phytopathogenic Pseudomonads and related plant-associated Pseudomonads. In Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E. eds The Prokaryotes: A Handbook on the Biology of Bacteria New York: Springer; 2006 pp 714–740
     [Google Scholar]
  8. Preston GM. Plant perceptions of plant growth-promoting Pseudomonas. Philos Trans R Soc Lond B Biol Sci 2004; 359:907–918 [View Article] [PubMed]
     [Google Scholar]
  9. Höfte M, De P. Plant perceptions of plant growth-promoting Pseudomonas species. In Plant-Associated Bacteria Springer; 2007 pp 507–533
     [Google Scholar]
  10. Swingle DB. Center rot of ‘french endive’ or wilt of chicory (Cichorium Intybus L.). Phytopathology 1925; 15:730
     [Google Scholar]
  11. Burgess SM, Lawson OB, Miller JW. Leafspot and blight of basil, Ocimum basilicum, caused by Pseudomonas cichorii. Proc Fla State Hort Soc 1986; 99:249–251
     [Google Scholar]
  12. Paula Wilkie J, Dye DW. Pseudomonas cichorii causing tomato and celery diseases in New Zealand. New Zealand J Agri Res 1974; 17:123–130 [View Article]
     [Google Scholar]
  13. Jones JB, Engelhard AW, Raju BC. Outbreak of a stem necrosis on chrysanthemum incited by Pseudomonas cichorii in florida. Plant Dis 1983; 67:431 [View Article]
     [Google Scholar]
  14. Grogan RG, Misaghi IJ, Kimble KA, Greathead AS, Ririe D. Varnish spot, destructive disease of lettuce in California caused by Pseudomonas cichorii. Phytopathology 1977; 77:957 [View Article]
     [Google Scholar]
  15. Engelhard AW, Mellinger HC, Ploetz RC, Miller JW. A leaf spot of florist’s geranium incited by Pseudomonas cichorii. Plant Dis 1983; 67:541–544
     [Google Scholar]
  16. Keinath AP, Wintermantel WM, Zitter TA. eds Compendium of Cucurbit Diseases and Pests, 2nd ed St. Paul: APS Press; 2018 [View Article]
     [Google Scholar]
  17. Patel N, Patel R, Wyenandt CA, Kobayashi DY. First report of Pseudomonas cichorii causing bacterial leaf spot on romaine lettuce (Lactuca sativa var. longifolia) and escarole (Cichorium endivia) in new jersey. Plant Dis 2021 [View Article]
     [Google Scholar]
  18. Miller JW, Knauss JF. Bacterial blight of Gerbera jamesonii incited by Pseudomonas cichorii. Plant Dis Reptr 1973; 57:504–505
     [Google Scholar]
  19. Yu SM, Lee YH. First report of Pseudomonas cichorii associated with leaf spot on soybean in South Korea. Plant Dis 2012; 96:142
     [Google Scholar]
  20. Lan DY, Shu FL, Lu YH, Shou AF, Lin W et al. First report of a leaf spot disease of tobacco caused by Pseudomonas cichorii in China. Plant Dis 2022; 106:1058 [View Article] [PubMed]
     [Google Scholar]
  21. McFadden LA. A bacterial leaf spot of florist’s chrysanthemums, Chrysanthemum morifolium. Plant Dis Rep 1961; 45:16
     [Google Scholar]
  22. Cottyn B, Heylen K, Heyrman J, Vanhouteghem K, Pauwelyn E et al. Pseudomonas cichorii as the causal agent of midrib rot, an emerging disease of greenhouse-grown butterhead lettuce in flanders. Syst Appl Microbiol 2009; 32:211–225
     [Google Scholar]
  23. Piening LJ, MacPherson DJ. Stem melanosis, a disease of spring wheat caused by Pseudomonas cichorii. Can J Plant Pathol 1985; 7:168–172 [View Article]
     [Google Scholar]
  24. Jang YW, Yoon YN, Maharjan R, Yi HJ, Jung MH et al. First report of Pseudomonas cichorii Causing bacterial vein necrosis on Perilla plants in South Korea. Plant Dis 2023; 107:549 [View Article]
     [Google Scholar]
  25. Goh R-P, Lee S, Fang Z-Q, Tang W-C, Chu C-C. First report of Pseudomonas cichorii causing bacterial leaf blight of pocketbook plant (Calceolaria hybrida) in Taiwan. Plant Dis 2024 [View Article] [PubMed]
     [Google Scholar]
  26. Patel N, Kobayashi DY, Noto AJ, Baldwin AC, Simon JE et al. First report of Pseudomonas cichorii causing bacterial leaf spot on sweet basil. Plant Dis 2019; 103: [View Article]
     [Google Scholar]
  27. Ahmad T, Guoqiang L, Wang J, Mingxuan L, Xiao Y et al. First report of bacterial leaf spot of Ficus benghalensis caused by Pseduomonas cichorii in pakistan. Plant Dis 2023 [View Article]
     [Google Scholar]
  28. Luiz BC, Villalun M, Eyre M, Bushe BC, Brill E et al. First report of bacterial leaf spot caused by Pseduomonas cichorii on monstera adansonii in hawai’i, USA. Plant Dis 2024 [View Article]
     [Google Scholar]
  29. Bull CT, Koike ST. Practical benefits of knowing the enemy: modern molecular tools for diagnosing the etiology of bacterial diseases and understanding the taxonomy and diversity of plant-pathogenic bacteria. Annu Rev Phytopathol 2015; 53:157–180 [View Article] [PubMed]
     [Google Scholar]
  30. Gomila M, Peña A, Mulet M, Lalucat J, García-Valdés E. Phylogenomics and systematics in Pseudomonas. Front Microbiol 2015; 6:214 [View Article] [PubMed]
     [Google Scholar]
  31. Gevers D, Cohan FM, Lawrence JG, Spratt BG, Coenye T et al. Opinion: re-evaluating prokaryotic species. Nat Rev Microbiol 2005; 3:733–739 [View Article] [PubMed]
     [Google Scholar]
  32. Fullem KR, Pena MM, Potnis N, Goss EM, Minsavage GV et al. Unexpected diversity of Pseudomonas associated with bacterial leaf spot of cucurbits in the Southeastern United States. Plant Dis 2024; 108:592–598 [View Article] [PubMed]
     [Google Scholar]
  33. Zhao M, Koirala S, Chen H-C, Gitaitis R, Kvitko B et al. Pseudomonas capsici sp. nov., a plant-pathogenic bacterium isolated from pepper leaf in Georgia, USA. Int J Syst Evol Microbiol 2021; 71: [View Article]
     [Google Scholar]
  34. Lu C-H, Han T-H, Jiang N, Gai X-T, Cao Z-H et al. Pseudomonas lijiangensis sp. nov., a novel phytopathogenic bacterium isolated from black spots of tobacco. Int J 2022; 72: [View Article]
     [Google Scholar]
  35. Zhao M, Gitaitis R, Dutta B. Characterization of Pseudomonas capsici strains from pepper and tomato. Front Microbiol 2023; 14:1267395 [View Article] [PubMed]
     [Google Scholar]
  36. Lelliott RA, Billing E, Hayward AC. A determinative scheme for the fluorescent plant pathogenic Pseudomonas. J Appl Bacteriol 1966; 29:470–489 [View Article] [PubMed]
     [Google Scholar]
  37. Obradovic A, Arsenijevic M. First report of a wilt and stem rot of muskmelon and watermelon transplants incited by Pseudomonas cichorii in Serbia. Plant Dis 2002; 86:443 [View Article]
     [Google Scholar]
  38. Schaad NW, Jones JB, Chun W. eds Laboratory Guide for Identification of Plant Pathogenic Bacteria, 3rd ed St. Paul: APS Press; 2001
     [Google Scholar]
  39. Prjibelski A, Antipov D, Meleshko D, Lapidus A, Korobeynikov A. Using SPAdes de novo assembler. Curr Protoc Bioinformatics 2020; 70:e102 [View Article] [PubMed]
     [Google Scholar]
  40. Berge O, Monteil CL, Bartoli C, Chandeysson C, Guilbaud C et al. A user’s guide to A data base of the diversity of Pseudomonas syringae and its application to classifying strains in this phylogenetic complex. PLoS One 2014; 9:e105547 Epub ahead of print 2014 [View Article] [PubMed]
     [Google Scholar]
  41. Gomila M, Busquets A, Mulet M, García-Valdés E, Lalucat J. Clarification of Taxonomic Status within the Pseudomonas syringae Species Group Based on a Phylogenomic Analysis. Front Microbiol 2017; 8:2422 [View Article] [PubMed]
     [Google Scholar]
  42. Davis EW, Weisberg AJ, Tabima JF, Grunwald NJ, Chang JH. Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria. PeerJ PreprintseerJ [View Article]
     [Google Scholar]
  43. Nguyen L-T, Schmidt HA, von Haeseler A, Minh BQ. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 2015; 32:268–274 [View Article] [PubMed]
     [Google Scholar]
  44. Letunic I, Bork P. Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation. Bioinformatics 2007; 23:127–128 [View Article] [PubMed]
     [Google Scholar]
  45. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014; 30:2068–2069 [View Article]
     [Google Scholar]
  46. Page AJ, Cummins CA, Hunt M, Wong VK, Reuter S et al. Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 2015; 31:3691–3693 [View Article] [PubMed]
     [Google Scholar]
  47. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. Bioinformatics 2013; 14:60 [View Article] [PubMed]
     [Google Scholar]
  48. Meier-Kolthoff JP, Carbasse JS, Peinado-Olarte RL, Göker M. TYGS and LPSN: A database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes. Nucleic Acids Res 2022; 50:D801–D807 [View Article] [PubMed]
     [Google Scholar]
  49. Pritchard L, Glover RH, Humphris S, Elphinstone JG, Toth IK. Genomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens. Anal Methods 2016; 8:12–24 [View Article]
     [Google Scholar]
  50. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article] [PubMed]
     [Google Scholar]
  51. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article] [PubMed]
     [Google Scholar]
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Pseudomonas serbiensis sp. nov. isolated from watermelon and muskmelon in Serbia
Int J Syst Evol Microbiol 74, 006613 (2024); https://doi.org/10.1099/ijsem.0.006613
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