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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

sp. nov., a Al-tolerant bacterium with the ability to inhibit f. sp. isolated from rhizosphere soil of muskmelon No Access

Abstract

A bacterial strain, designated as A6, was isolated from the rhizosphere soil of a healthy muskmelon in Wenchang, Hainan Province, China. The cells of strain A6 were Gram-negative, aerobic, short rod and motile with a single polar flagellum. Strain A6 could tolerate up to 55.0 mM Al and inhibited the growth of f. sp. which is the pathogen of muskmelon . Growth occurred at 15–37 ℃ (optimum at 30 ℃), pH 4.5–8.0 (optimum pH 6.5) and with 0–3.0 % NaCl (w/v; optimum, 0.5%). Strain A6 shared the highest 16S rRNA gene sequence similarities with Sa (98.0%), followed by ATSB10 (98.0%), 5GH9-34 (97.9%), DHG59 (97.7%), DHo (97.7%) and 5GH9-11 (97.7%). Phylogenetic trees based on 16S rRNA gene and genomic sequences indicated that strain A6 belonged to the genus and formed a subclade with Sa and ATSB10. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) values between A6 and its closely related type strains were 78.8–80.8 %, 70.0–71.7 % and 20.5–22.1 %, respectively. The sole respiratory quinone was ubiquinone-8 (Q-8). The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL1-2) and three unidentified phospholipids (PL1-3). The major cellular fatty acids (≥5 %) were iso-C, C, summed feature 9 (iso-C  9 and/or C 10-methyl), iso-C, iso-C and anteiso-C. The genome size of strain A6 was 3.7 Mb with a DNA G+C content of 65.1%. Based on the phenotypic, phylogenetic, genotypic and chemotaxonomic features, strain A6 represents a novel species in the genus , for which A6 sp. nov. is proposed. The type strain is A6 (= GDMCC 1.4640 = KCTC 92542).

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Keyword(s): chemotaxonomic characterization , Dyella aluminiiresistens sp. nov , phylogeny and polyphasic taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41701307)
    • Principal Award Recipient: YiLi
  • National Natural Science Foundation of China (Award 42177402)
    • Principal Award Recipient: ChunyuanWu
  • Hainan Province Science and Technology Special Fund (Award ZDYF2021XDNY188)
    • Principal Award Recipient: YiLi
© 2025 The Authors
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2025-01-06
2026-02-17

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Dyella aluminiiresistens sp. nov., a Al3+-tolerant bacterium with the ability to inhibit Fusarium oxysporum f. sp. melonis isolated from rhizosphere soil of muskmelon
Int J Syst Evol Microbiol 75, 006611 (2025); https://doi.org/10.1099/ijsem.0.006611
/content/journal/ijsem/10.1099/ijsem.0.006611
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