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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 12

sp. nov., isolated from Darangshi Oreum (a volcanic cone), and the reclassification of Lu . 2003 as a later heterotypic synonym of (Shinobu 1962) Preobrazhenskaya . 1975 (Approved Lists 1980) No Access

Abstract

Two mycelium-forming actinobacterial strains, designated as DLS-47 and DLS-62, were isolated from volcanic ash collected from the surface of a rock on the peak of Darangshi Oreum (a volcanic cone) in Jeju, Republic of Korea, and their taxonomic positions were investigated by a polyphasic approach. Both of the isolates showed growth at 20–42 °C, pH 6.0–9.0 and 0–1% (w/v) NaCl. Furthermore, DLS-47 was found to grow at 45 °C, while strain DLS-62 grew at pH 10.0 and 3% (w/v) NaCl. The 16S rRNA gene-based phylogeny showed that both of the isolates belonged to the genus ; strain DLS-47 was most closely related to DSM 45346 (100% sequence identity), while strain DLS-62 formed a tight cluster with DSM 45347 (99.5% sequence similarity). Morphological and chemotaxonomic characteristics supported the affiliation of the two isolates to the genus . Phylogenomic analysis based on 92 core gene sequences showed that both of the isolates were most closely related to DSM 45346. Strain DLS-47 shared 100% of orthologous average nucleotide identity and digital DNA–DNA hybridization values with DSM 45346, while strain DLS-62 showed orthologous average nucleotide identity ≤89.8% and digital DNA–DNA hybridization values ≤39.4% with strain DLS-47 and members of the genus . The results of phenotypic assays and comparison of overall genomic relatedness indices support the conclusion that strain DLS-47 (= KACC 23347=DSM 116423) is a strain of , while strain DLS-62 (= KACC 23345 = DSM 116424) represents a new species of the genus , for which the name sp. nov. is proposed. Also, Lu 2003 is reclassified as a later heterotypic synonym of (Shinobu 1962) Preobrazhenskaya . 1975 (Approved Lists 1980) based on analysis of overall genomic relatedness indices and phenotypic similarity.

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Keyword(s): Actinomadura glauciflava , Actinomadura luteofluorescens , Actinomadura monticuli sp. nov. , heterotypic synonym , volcanic ash and volcanic cone
Funding
This study was supported by the:
  • BioPS Co, Ltd.
    • Principal Award Recipient: SoonDong Lee
© 2024 The Authors
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Actinomadura monticuli sp. nov., isolated from Darangshi Oreum (a volcanic cone), and the reclassification of Actinomadura glauciflava Lu et al. 2003 as a later heterotypic synonym of Actinomadura luteofluorescens (Shinobu 1962) Preobrazhenskaya et al. 1975 (Approved Lists 1980)
Int J Syst Evol Microbiol 74, 006609 (2024); https://doi.org/10.1099/ijsem.0.006609
/content/journal/ijsem/10.1099/ijsem.0.006609
/content/journal/ijsem/10.1099/ijsem.0.006609
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