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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 1

sp. nov., isolated from duckweed and formal proposal to reclassify as a later heterotypic synonym of and reclassify as subsp. No Access

Abstract

A novel strain DW16-2, isolated from duckweed (), was taxonomically studied in detail. The analysis based on its 16S rRNA gene sequence revealed that the strain was most closely related to Y8 (98.8%), followed by YIM 61452 (98.7%), DVS 5a1 (98.7%) and DSM 44104 (98.7%). The average nucleotide identity (ANI) based on and digital DNA–DNA hybridization (dDDH) relatedness values between strain DW16-2 and their closest type strains were below the threshold values for identifying a novel species. Morphological, physiological and chemotaxonomic features of strain DW16-2 were typical for the genus by forming extensively branched substrate mycelium and aerial mycelium that fragmented into rod-shaped spore, with a smooth surface. The whole-cell hydrolysates of strain DW16-2 contained -diaminopimelic acid as the diagnostic diamino acid, and the whole-cell sugars were arabinose, galactose, glucose and a trace amount of ribose. The polar lipids contained phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and unidentified phospholipids. The menaquinone (MK) was MK-8(H). The cellular fatty acids (>5 %) were -C, -C H, summed feature 3: C ω7c/C ω6c; C ω6c/C ω7c, C ω8c and -C. Characterization based on chemotaxonomic, phenotypic, genotypic and phylogenetic evidence demonstrated that strain DW16-2 represents a novel species of the genus , for which the name sp. nov. (type strain DW16-2 = TBRC 16418 = NBRC 115857) is proprosed. In addition, the comparison of the whole genome sequences suggested that and belong to the same species and is a subspecies of . Therefore, it is proposed that Prabahar . 2004 is reclassified as a later heterotypic synonym of (Evtushenko . 1989) Warwick . 1994, and Park . 2008 is proposed as a subspecies of (Evtushenko . 1989) Warwick . 1994.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): actinomycete , polyphasic taxonomy , Pseudonocardia and Spirodela polyrhiza
Funding
This study was supported by the:
  • Graduate Program Scholarship from The Graduate School, Kasetsart University
    • Principal Award Recipient: WaranyaButdee
© 2025 The Authors
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2025-01-03
2025-11-16

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Pseudonocardia spirodelae sp. nov., isolated from duckweed and formal proposal to reclassify Pseudonocardia antarctica as a later heterotypic synonym of Pseudonocardia alni and reclassify Pseudonocardia carboxydivorans as Pseudonocardia alni subsp. carboxydivorans
Int J Syst Evol Microbiol 75, 006608 (2025); https://doi.org/10.1099/ijsem.0.006608
/content/journal/ijsem/10.1099/ijsem.0.006608
/content/journal/ijsem/10.1099/ijsem.0.006608
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