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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 12

sp. nov., a member of the isolated from badland surfaces in the Red Desert, Wyoming, USA Open Access

Abstract

A novel Gram-stain-positive, black-pigmented bacterium, designated as WL48A , was isolated from the surface of badland sedimentary rock in the Red Desert of Wyoming and characterized using a polyphasic taxonomic approach. Good growth occurred at 28–32 °C, pH 7–9, and NaCl less than 1% (w/v). Colonies, growing well on International Streptomyces Project media (ISP) 3 and ISP 7, were black and adhering to the agar. Phylogenetic analyses based on 16S rRNA gene and draft genome sequences showed that strain WL48A belongs to the family , forming a distinct sub-branch with DSM 46841. The organism showed 16S rRNA gene sequence similarity of 98.8% with DSM 46841. Digital DNA–DNA hybridization value between the genome sequences of strain WL48A and DSM 46841 was 51.8%, below the threshold of 70% for prokaryotic species delineation. The chemotaxonomic investigation revealed the presence of galactose, glucose, mannose, xylose and ribose as well as -DAP in the peptidoglycan layer. The polar lipid profiles contained phosphatidylcholine (PC), phosphatidylinositol (PI), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) phosphoglycolipid, phospholipids and an unidentified lipid. The menaquinone profile consisted of MK-9(H) (98.2%) and MK-9(H) (10.8%). The major fatty acid profile (>15%) comprised iso-C and iso-C. Based on phenotypic, genetic and genomic data, strain WL48A (=DSM 116197 = NCIMB 15483=NCCB 100957 =ATCC TSD-376) merits to be considered as a novel species for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Geodermatophilaceae , phylogenetic analysis , phylogenomic analysis , polyphasic taxonomy and Wyoming badland formation
Funding
This study was supported by the:
  • EPSCoR (Award EPS-1655726)
    • Principal Award Recipient: NotApplicable
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-12-13
2026-01-17

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Geodermatophilus maliterrae sp. nov., a member of the Geodermatophilaceae isolated from badland surfaces in the Red Desert, Wyoming, USA
Int J Syst Evol Microbiol 74, 006603 (2024); https://doi.org/10.1099/ijsem.0.006603
/content/journal/ijsem/10.1099/ijsem.0.006603
/content/journal/ijsem/10.1099/ijsem.0.006603
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