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Volume 74, Issue 11

Genomic profiling and assessment of the plant growth-enhancing traits in the novel actinomycete, sp. nov. No Access

Abstract

Strain KLBMP 9567, an isolate from weathered soil, was identified as a new actinobacterial species through a comprehensive polyphasic approach. Phylogenetic evaluations relying on 16S rRNA gene sequence placed KLBMP 9567 within the genus , alongside its close relatives NEAU-FHS4 and NEAU-QY2, both sharing 98.6% sequence similarity. However, KLBMP 9567 demonstrated low digital DNA–DNA hybridization values with NEAU-FHS4 and NEAU-QY2, recorded at 48.5 and 29.1%, respectively. Meanwhile, the average nucleotide identity values were correspondingly recorded at 91.1 and 83.1%. The cell wall of KLBMP 9567 contained -diaminopimelic acid, with xylose, mannose, glucose and galactose as diagnostic sugars. The diagnostic dominant phospholipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Given phenotypic and genotypic data, KLBMP 9567 is recognized as a new species within the genus, named sp. nov., with the type strain KLBMP 9567 (= CGMCC 4.7773 = NBRC 115787). Genome mining revealed genes associated with plant growth promotion, specifically those encoding enzymes for synthesizing plant hormones like indole acetic acid and gibberellic acid. Experimental validation demonstrated that KLBMP 9567 can produce significant quantities of indole acetic acid (20.6 mg l) and gibberellic acid (42.6 mg l). Moreover, co-culture with showed marked growth enhancements: a 56.3% increase in lateral root proliferation, a 16.7% elongation in primary root length and a 44.8% boost in biomass accumulation. These findings underscore the strain’s potent plant growth-promoting attributes, providing profound insights into the mechanisms by which KLBMP 9567 enhances plant growth.

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  • Accepted:
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Keyword(s): genome analysis , hormone biosynthesis , Micromonosporaceae , plant growth promotion and polyphasic taxonomy
Funding
This study was supported by the:
  • Postgraduate Research & Practice Innovation Program of Jiangsu Province (Award KYCX24_3023)
    • Principle Award Recipient: LiliWang
  • National College Students Innovation and Entrepreneurship Training Program (Award 202310320003Z)
    • Principle Award Recipient: ZhouaiMa
  • National Natural Science Foundation of China (Award 32030072)
    • Principle Award Recipient: JihongJiang
© 2024 The Authors
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2024-11-26
2024-12-08
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Genomic profiling and assessment of the plant growth-enhancing traits in the novel actinomycete, Plantactinospora soli sp. nov.
Int J Syst Evol Microbiol 74, 006590 (2024); https://doi.org/10.1099/ijsem.0.006590
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/content/journal/ijsem/10.1099/ijsem.0.006590
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