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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 12

Unveiling three novel actinobacterial species ( sp. nov., sp. nov. and sp. nov.) in soil samples in Phu Pha Phet Cave, Thailand No Access

Abstract

Three strains of actinobacteria, designated as B-S-A6, B-S-A8 and B-S-A12, were isolated from soil samples collected in the Phu Pha Phet Cave located in the Satun UNESCO Global Geopark, Satun Province, Thailand. A comprehensive polyphasic approach was used to describe these strains. Strains B-S-A6, B-S-A8 and B-S-A12 were identified within the genus based on a comparative examination of 16S rRNA gene sequences. Strains B-S-A8 and B-S-A12 showed a high genetic similarity to NBRC 13424 (99.0%, for both) and to C63 (98.6 and 98.7 %, respectively). Meanwhile, strain B-S-A6 exhibited 98.6% sequence similarity with NBRC 13424 and VK-A6 . However, upon further comparison, the 16S rRNA gene sequence of strain B-S-A6 showed a similarity of 99.6% to B-S-A8 and 99.7% to B-S-A12. Contrastingly, B-S-A8 showed a very high similarity of 99.8% with B-S-A12. The use of digital DNA–DNA hybridization, along with the analysis of average nt identities and aa identities, comparing the three strains to their closest known type strains, validated that each of these strains represents a new and distinct species. Three newly identified strains exhibited phenotypic characteristics and chemotaxonomic properties consistent with the members of the genus . The primary menaquinones identified in these strains were MK-9(H) and MK-9(H). The major fatty acids were -C and -C. Their polar lipid profiles included diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The comprehensive phenotypic and genomic analyses of the strains strongly suggest that strains B-S-A6, B-S-A8 and B-S-A12 represent three new species for which the names sp. nov. (type strain B-S-A6 =TBRC 17074 =NBRC 116118), sp. nov. (type strain B-S-A8 =TBRC 17072 =NBRC 116117) and sp. nov. (type strain B-S-A12 =TBRC 17060 =NBRC 116116) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinomycetes , cave , Streptomyces cavernicola , Streptomyces luteolus , Streptomyces solicavernae and Thailand
Funding
This study was supported by the:
  • RDI Management for National Strategic and Network Division–NSTDA (Award P1951709 and P2250739)
    • Principal Award Recipient: NitchaChamroensaksri
© 2024 The Authors
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2024-12-09
2026-01-22

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/content/journal/ijsem/10.1099/ijsem.0.006563
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Unveiling three novel actinobacterial species (Streptomyces cavernicola sp. nov., Streptomyces solicavernae sp. nov. and Streptomyces luteolus sp. nov.) in soil samples in Phu Pha Phet Cave, Thailand
Int J Syst Evol Microbiol 74, 006563 (2024); https://doi.org/10.1099/ijsem.0.006563
/content/journal/ijsem/10.1099/ijsem.0.006563
/content/journal/ijsem/10.1099/ijsem.0.006563
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