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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 12

sp. nov., a Gram-positive mesophilic bacterium isolated from plant lesions No Access

Abstract

A bacterial strain was isolated from pathogenic lesions of tree leaves from the Teutoburg Forest in North Rhine-Westphalia, Germany, by culture on non-selective agar plates. 16S rRNA sequencing revealed 100% similarity to and , as well as 99% similarity to and . Here, we used genome-based taxonomy with the Type (Strain) Genome Server (TYGS), which suggests the isolation of a novel prokaryotic strain. According to TYGS-analysis, using whole genome digital DNA–DNA hybridization, only 65.5% similarity to the closest relative was revealed, suggesting a novel species. Growth was observed at both aerobic and anaerobic conditions. Bacterial cells depicted coryneform motile rods, with a length of 1.1–3.3 µm and a constant diameter of 0.5 µm. Cells did not form spores under the tested conditions and stain Gram-positive. Growth occurred between 0.5 and 4% NaCl (optimal: 1%), at pH 5.5–9.5 (optimal: 8.0–9.0). The strain was mesophilic with an optimal growth at 25°C. Major cellular fatty acids of the novel strain were anteiso-C and C.

  • Received:
  • Accepted:
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Keyword(s): 16S rRNA , Acer campestre , Bielefeld , mesophilic , novel species , plant leave , plant lesion , Sanguibacter biliveldensis and Teutoburg Forest
Funding
This study was supported by the:
  • Universität Bielefeld
    • Principal Award Recipient: ChristianKaltschmidt
© 2024 The Authors
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2024-12-03
2025-11-11

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/content/journal/ijsem/10.1099/ijsem.0.006560
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Sanguibacter biliveldensis sp. nov., a Gram-positive mesophilic bacterium isolated from plant lesions
Int J Syst Evol Microbiol 74, 006560 (2024); https://doi.org/10.1099/ijsem.0.006560
/content/journal/ijsem/10.1099/ijsem.0.006560
/content/journal/ijsem/10.1099/ijsem.0.006560
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