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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 10

sp. nov., a novel protease-producing and cellulose-degrading actinobacterium isolated from soil No Access

Abstract

A novel protease-producing and cellulose-degrading actinobacterium, designated strain NEAU-NG30, was isolated from a melon rhizosphere soil sample collected in Harbin, Heilongjiang Province, China, and established its status using a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-NG30 was closely related to Amycolatopsis bullii DSM 45802 (98.7%) and Amycolatopsis vancoresmycina DSM 44592 (98.3%). The phospholipid profile contained diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylinositol. The diagnostic sugars in cell hydrolysates were determined to be galactose and arabinose. Cell walls contained -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-9(H). The major fatty acids were iso-C and iso-C. Meanwhile, genome analysis of sequences revealed a genome size of 9 338 250 bp and a DNA G+C content of 71.6%. In addition, the average nucleotide identity values and the level of digital DNA–DNA hybridization between strain NEAU-NG30 and its reference strains fall below the thresholds typically used for delineating prokaryote species. According to phenotypic, chemotaxonomic and genotypic studies, it is indicated that strain NEAU-NG30 is considered to be a novel species of the genus , for which the name sp. nov. is proposed, with NEAU-NG30 (=MCCC 1K08677=JCM 35654) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , genome characterization , novel species and taxonomy
Funding
This study was supported by the:
  • State Key Program of National Natural Science Foundation of China (Award 32030090)
    • Principal Award Recipient: WenshengXiang
  • National Key Research and Development Program of China (Award 2023YFD1700700)
    • Principal Award Recipient: WenshengXiang
© 2024 The Authors
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2024-10-29
2025-11-09

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Amycolatopsis melonis sp. nov., a novel protease-producing and cellulose-degrading actinobacterium isolated from soil
Int J Syst Evol Microbiol 74, 006559 (2024); https://doi.org/10.1099/ijsem.0.006559
/content/journal/ijsem/10.1099/ijsem.0.006559
/content/journal/ijsem/10.1099/ijsem.0.006559
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