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Abstract

An endosymbiotic bacterium of the genus , designated as strain HZ, was cultured from the parasitoid wasp , which develops on the pupae of various host flies. The bacterium was detected in developed on houseflies, , in a poultry facility in Hazon, northern Israel. After culturing, this bacterium displayed no surface motility on Luria–Bertani agar and was rod-shaped and irregular in size, ~10–30 nm in diameter and 5–20 µm in length. Phylogenetic analyses revealed that strain HZ is closely related to strain HS, a free-living species of the genus that includes many insect endosymbionts. Although these bacteria maintain >98% sequence identity in shared genes, genomic characterization revealed that strain HZ has undergone substantial reductive evolution, such that it lacks many gene functions that are maintained in strain HS. Based on the results of phylogenetic, genomic and chemotaxonomic analyses, we propose that this endosymbiont should be classified in a new subspecies as subsp. s subsp. nov. The type strain for this new subspecies is HZ (=ATCC TSD-398=NCIMB 15482). The subspecies subsp. strain HS is created automatically with the type strain ATCC BAA-2554 (=DSMZ 27494).

Funding
This study was supported by the:
  • Binational Science Foundation (Award 2020791)
    • Principle Award Recipient: EladChiel
  • National Science Foundation (Award DEB 2114510)
    • Principle Award Recipient: ColinDale
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2024-10-28
2024-11-05
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