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Abstract

Three Gram-stain-negative, strictly aerobic, non-motile, oxidase- and catalase-positive, short-rod-shaped bacteria, designated as strains G8-12, SS1-5 and BS5-3, were isolated from marine algae in South Korea. Strain G8-12 exhibited optimal growth at 20–25 °C, pH 8.0 and 2.0–2.5% (w/v) NaCl, while strains SS1-5 and BS5-3 grew optimally at 25 °C, pH 7.0 and 1.5% NaCl. All strains contained ubiquinone-10 as the sole respiratory quinone, with phosphatidylglycerol and phosphatidylcholine as major polar lipids, and C 7 and C as major fatty acids (>5 %); C 7 11-methyl and C 2-OH were additionally identified as major fatty acids in strain SS1-5. The genomic DNA G+C contents were 57.0, 58.3 and 56.4% for strains G8-12, SS1-5 and BS5-3, respectively. Strains G8-12, SS1-5 and BS5-3 exhibited less than 74.8% average nucleotide identity (ANI) and 19.7% digital DNA–DNA hybridization (dDDH) values with each other, indicating that they represent different species. Phylogenetic analyses based on both 16S rRNA gene and genome sequences revealed that strains G8-12, SS1-5 and BS5-3 form distinct phylogenetic lineages within the genus . Relative to other closely related species, these strains exhibited ANI and dDDH values below 83.5 and 26.9%, respectively, suggesting that they constitute novel species within the genus . Based on their phenotypic, chemotaxonomic and phylogenetic characteristics, strains G8-12, SS1-5 and BS5-3 represent three novel species of the genus , for which the names sp. nov. (G8-12=KACC 22753=JCM 35790), sp. nov. (SS1-5=KACC 22649=JCM 35753) and sp. nov. (BS5-3=KACC 22648=JCM 35751) are proposed, respectively.

Funding
This study was supported by the:
  • Chung-Ang University (Award 2023)
    • Principle Award Recipient: JiHoon Jeon
  • National Institute of Biological Resources (Award NIBR No. 2023-02-001)
    • Principle Award Recipient: CheOk Jeon
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-10-16
2024-11-10
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