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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 9

sp. nov., isolated from river sediment No Access

Abstract

A Gram-stain-negative, non-spore-forming and strictly aerobic bacterial strain, designated R-7, was isolated from river sediment in Wuxi, Jiangsu, PR China. Cells (1.6–3.8 µm long and 0.6–0.8 µm wide) were slightly curved to straight rods and motile by means of a polar flagellum. The strain grew optimally on Reasoner's 2A medium at 30 °C, pH 7.0 and with 1.0% (w/v) NaCl. Strain R-7 exhibited closest 16S rRNA gene sequence similarities to CGMCC 1.7660 (95.4%), 04SU4-P (94.6%) and D78 (93.8%). The phylogenetic trees based on genomic and 16S rRNA gene sequences showed that strain R-7 was clustered in the genus . The obtained average nucleotide identity and digital DNA–DNA hybridization values between R-7 and the three type strains of the genus were 73.4, 72.8 and 72.4% and 19.5, 19.0 and 18.7%, respectively. The major respiratory quinone was Q-10. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminolipids, two unidentified aminophospholipids and nine unidentified polar lipids. The major cellular fatty acids (>5% of the total) were cyclo-C 8, C and C 2-OH. The DNA G+C content was 65.5 mol%. On the basis of the evidence presented in this study, strain R-7 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain R-7 (=KCTC 8082=MCCC 1K08805) as the type strain.

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Keyword(s): 16S rRNA gene , Dongia sedimenti sp. nov. and polyphasic taxonomy
Funding
This study was supported by the:
  • the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
    • Principal Award Recipient: JianHe
  • Special Fund for Scientific Innovation Strategy-Construction of High-level Academy of Agriculture Science (Award BM2022019)
    • Principal Award Recipient: JianHe
  • National Natural Science Foundation of PR China (Award 32170128)
    • Principal Award Recipient: JianHe
© 2024 The Authors
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2024-09-23
2025-11-17

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Dongia sedimenti sp. nov., isolated from river sediment
Int J Syst Evol Microbiol 74, 006532 (2024); https://doi.org/10.1099/ijsem.0.006532
/content/journal/ijsem/10.1099/ijsem.0.006532
/content/journal/ijsem/10.1099/ijsem.0.006532
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