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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 9

sp. nov., isolated from deep-sea sediments No Access

Abstract

Three Gram-stain-negative, aerobic, non-motile, chemoheterotrophic, short-rod-shaped bacteria, designated CDY1-MB1, CDY2-MB3, and BDY3-MB2, were isolated from three marine sediment samples collected in the eastern Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains were related to the genus and close to the type strain of F4716 (with similarities of 98.0–98.1%). Strain CDY1-MB1 can grow at 15–37 °C (optimum 30 °C) and in media with pH 6–9 (optimum, pH 7), and tolerate up to 10% (w/v) NaCl. The predominant cellular fatty acids of strain CDY1-MB1 were iso-C (20.7%) and iso-C 3-OH (12.8%); the sole respiratory quinone was menaquinone 6; the major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified polar lipids. The digital DNA–DNA hybridization/average nucleotide identity values between strains CDY1-MB1, CDY2-MB3, and BDY3-MB2 and F4716 were 24.7%/81.6–81.7%, thereby indicating that strain CDY1-MB1 should represent a novel species of the genus . The genomic DNA G+C contents were 37.6 % in all three strains. Genomic analysis showed the presence of genes related to nitrogen and sulphur cycling, as well as metal reduction. The genetic traits of these strains indicate their possible roles in nutrient cycling and detoxification processes, potentially shaping the deep-sea ecosystem’s health and resilience. Based upon the consensus of phenotypic and genotypic analyses, strain CDY1-MB1 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CDY1-MB1 (=MCCC 1A16935=KCTC 102223).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aequorivita , genome analysis , Pacific Ocean and phylogenetic analysis
Funding
This study was supported by the:
  • Open Funding Project of the Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University (Award 20220502, 20220501)
    • Principle Award Recipient: JinmeiXia
  • the Scientific Research Foundation of the Third Institute of Oceanography, MNR (Award 2019021)
    • Principle Award Recipient: ZongzeShao
  • Scientific Research Foundation of the Third Institute of Oceanography, MNR (Award 2022007)
    • Principle Award Recipient: JinmeiXia
  • the COMRA Program (Award DY135-B2-01)
    • Principle Award Recipient: ZongzeShao
  • National Key R&D Program (Award 2022YFC2804100)
    • Principle Award Recipient: JinmeiXia
© 2024 The Authors
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Aequorivita flava sp. nov., isolated from deep-sea sediments
Int J Syst Evol Microbiol 74, 006513 (2024); https://doi.org/10.1099/ijsem.0.006513
/content/journal/ijsem/10.1099/ijsem.0.006513
/content/journal/ijsem/10.1099/ijsem.0.006513
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