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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 8

Phytoplasma vignae’, assigning a species description to a long-known phytoplasma occurring in northern Australia Open Access

Abstract

Gene- and genome-based approaches were used to determine whether little leaf (ViLL) phytoplasma, which occurs in northern Australia, is a distinct ‘ Phytoplasma’ species. The ViLL 16S rRNA gene sequences exhibited the highest known similarity to species in the 16SrXXIX-A and 16SrIX-D subgroups, namely ‘ Phytoplasma omanense’ (98.03–98.10%) and ‘ Phytoplasma phoenicium’ (96.87–97.20%), respectively. A total of 48 single-copy orthologue genes were identified to be shared among the two draft ViLL phytoplasma genomes, 30 publicly available phytoplasma genomes, and one genome as the outgroup taxon. Phylogenomic assessments using the 48 shared single-copy orthologue genes supported that ViLL and ‘. Phytoplasma phoenicium’ were closely related yet distinct species. The 16S rRNA gene sequence analysis and phylogenomic assessment indicate that ViLL phytoplasmas are a distinct taxon. As such, a novel species, ‘ Phytoplasma vignae’, is proposed. Strain BAWM-336 (genome accession number JAUZLI000000000) detected in (bitter melon) serves as the reference strain of this species, with infected plant material deposited in the Victorian Plant Pathology Herbarium (VPRI) as VPRI 44369.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Mollicutes , phylogenomics , phytopathogen , Vigna little leaf phytoplasma and ViLL
Funding
This study was supported by the:
  • University of Tasmania
    • Principle Award Recipient: BiancaRodrigues Jardim
  • Department of Primary Industries and Regional Development, Government of Western Australia
    • Principle Award Recipient: BiancaRodrigues Jardim
  • Northern Territory Government
    • Principle Award Recipient: BiancaRodrigues Jardim
  • Department of Economic Development, Jobs, Transport and Resources, State Government of Victoria
    • Principle Award Recipient: BiancaRodrigues Jardim
  • Department of Agriculture and Fisheries, Queensland Government
    • Principle Award Recipient: BiancaRodrigues Jardim
  • Hort Innovation
    • Principle Award Recipient: BiancaRodrigues Jardim
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-08-27
2025-06-18
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'Candidatus Phytoplasma vignae’, assigning a species description to a long-known phytoplasma occurring in northern Australia
Int J Syst Evol Microbiol 74, 006502 (2024); https://doi.org/10.1099/ijsem.0.006502
/content/journal/ijsem/10.1099/ijsem.0.006502
/content/journal/ijsem/10.1099/ijsem.0.006502
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