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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 8

sp. nov., isolated from human upper respiratory samples collected in Botswana No Access

Abstract

Four strains (MSK211, MSK294, MSK312, MSK433) of a novel species were cultured from nasopharyngeal swabs collected from mother–infant dyads in southern Botswana. These strains grew optimally on tryptic soy agar with 5% sheep blood solid medium and in fastidious bacteria broth. Colonies on tryptic soy agar with 5% sheep blood agar appeared grey or white with a flat, smooth surface and variable alpha haemolysis. Cells were Gram-positive, non-spore-forming, non-motile cocci that lacked catalase or oxidase activity. Major fatty acids were C (palmitic acid), Cω9 (oleic acid), and C (stearic acid). Analyses of 16S rRNA gene sequences identified these strains as belonging to the genus (family ), which currently contains only a single validly published species (). Whole-genome sequencing revealed that the genomes of these strains are 1.98–2.07 Mbp in size and have a G+C content of 39.6–39.9 mol%. Comparisons of these genomes to publicly available genomes of yielded average nucleotide identities and DNA–DNA hybridization values of 92.3–92.9% and 49.1–51.4%, respectively. These results indicate that these strains represent a novel species of , for which we propose the name sp. nov., with the type strain MSK294 (=DSM 117171=JCM 36673).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Carnobacteriaceae , infant nasal cavity , lactic acid bacterium , nasopharyngeal microbiota and whole-genome sequencing
Funding
This study was supported by the:
  • Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases (Award K01- AI173398)
    • Principle Award Recipient: JillianH. Hurst
  • Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases (Award K23-AI135090)
    • Principle Award Recipient: MatthewS. Kelly
© 2024 The Authors
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2024-08-21
2024-09-15
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Dolosigranulum savutiense sp. nov., isolated from human upper respiratory samples collected in Botswana
Int J Syst Evol Microbiol 74, 006498 (2024); https://doi.org/10.1099/ijsem.0.006498
/content/journal/ijsem/10.1099/ijsem.0.006498
/content/journal/ijsem/10.1099/ijsem.0.006498
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