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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 8

sp. nov., sp. nov., sp. nov. and sp. nov., isolated from streams in China and re-examining the taxonomic status of all the genera within the family No Access

Abstract

Four Gram-stain-negative, aerobic, rod-shaped and motile strains (LYT19W, DXS22W, DXS29W and BYS139W) were isolated from streams in China. All four strains showed highest 16S rRNA gene sequence similarities to the species of genus . The calculated average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values among strains LYT19W, DXS22W, DXS29W, BYS139W and other closely related strains were less than 79.5, 22.5 and 74.0%, respectively, indicating that each of the four strains should represent an independent novel species. The further reconstructed phylogenomic tree showed that strains LYT19W and DXS29W clustered closely with strains, but strains DXS22W and BYS139W formed an independent clade with and . Comparing with other species, and harboured distinct physiological and genetic characteristics, so it was reasonable to propose a novel genus to accommodate , , DXS22W and BYS139W. The major fatty acids of the four strains contained C, summed feature 3 (C 6 and/or C 7) and summed feature 8 (C 6 and/or C 7). The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and one unidentified aminophospholipid. Combining above descriptions, strains LYT19W and DXS29W should represent two novel species of the genus , for which the names sp. nov. (type strain LYT19W=GDMCC 1.3205=KCTC 92545) and sp. nov. (type strain DXS29W=GDMCC 1.3207=KCTC 92547) are proposed, respectively. Strains DXS22W and BYS139W should represent two novel species of the new genus , for which the names sp. nov. (type strain DXS22W=GDMCC 1.3206=KCTC 92546) and sp. nov. (type strain BYS139W=GDMCC 1.3208=KCTC 92548) are proposed, respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aquabacterium , Ideonella and streams
Funding
This study was supported by the:
  • Yunnan Provincial Ministry of Science and Technology (Award 202301AT070100)
    • Principle Award Recipient: HuibinLu
  • Yunnan Provincial Ministry of Science and Technology (Award 202203AC100002-02, 202305AM070002)
    • Principle Award Recipient: LiChen
  • Science and Technology Projects of Southwest Joint Graduate School of Yunnan Province (Award 202302AP370001)
    • Principle Award Recipient: LiChen
© 2024 The Authors
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2024-08-28
2024-09-15
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Ideonella margarita sp. nov., Ideonella lacteola sp. nov., Pseudaquabacterium inlustre sp. nov. and Pseudaquabacterium rugosum sp. nov., isolated from streams in China and re-examining the taxonomic status of all the genera within the family Sphaerotilaceae
Int J Syst Evol Microbiol 74, 006495 (2024); https://doi.org/10.1099/ijsem.0.006495
/content/journal/ijsem/10.1099/ijsem.0.006495
/content/journal/ijsem/10.1099/ijsem.0.006495
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