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Abstract

Two Gram-negative, obligately aerobic, rod-shaped bacteria, strains G1-22 and G1-23, were isolated from the phycosphere of a marine brown alga. Both strains exhibited catalase- and oxidase-positive activities. Strain G1-22 displayed optimal growth at 25 °C, pH 8.0, and 2.0–3.0% (w/v) NaCl, while strain G1-23 exhibited optimal growth at 25 °C, pH 8.0, and 4.0% NaCl. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. As major fatty acids (> 5%), strain G1-22 contained C, summed feature 3 (C 7 and/or C 6), summed feature 8 (C 7 and/or C 6), C 3-OH, and C 3-OH, while strain G1-23 contained C, summed feature 3 (C 7 and/or C 6), and C. Phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were major polar lipids in both strains. Strains G1-22 and G1-23 had DNA G+C contents of 40.2 and 38.9 mol%, respectively. Phylogenetic analyses based on 16S rRNA and genome sequences revealed that strains G1-22 and G1-23 formed distinct phylogenetic lineages within the genera and , respectively. Strain G1-22 showed closest relatedness to MTZ26 with 97.8% 16S rRNA gene sequence similarity, 70.2% average nucleotide identity (ANI), and a 21.5% digital DNA–DNA hybridization (dDDH) value, while strain G1-23 was most closely related to KCTC 32108 with 95.6% 16S rRNA gene sequence similarity, 74.6% ANI, and a 20.1% dDDH value. Based on phenotypic and molecular characteristics, strains G1-22 and G1-23 are proposed to represent two novel species, namely sp. nov. (type strain G1-22=KACC 22486=JCM 34971) and sp. nov. (type strain G1-23=KACC 22490=JCM 34972), respectively. Additionally, based on the comparison of whole genome sequences, it is proposed that , , and are reclassified as later heterotypic synonyms of , , and , respectively.

Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR No. 2024-02-001)
    • Principle Award Recipient: CheOk Jeon
  • Ministry of Oceans and Fisheries (Award 20210469)
    • Principle Award Recipient: CheOk Jeon
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-08-14
2024-11-10
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