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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 8

Two new members of the genus : sp. nov. and sp. nov. No Access

Abstract

Four strains, designated dk4302, dk4209, xlx-73, and xlx-183, were isolated from Tibetan gazelle and red swamp crawfish collected from the Qinghai–Tibet Plateau and Jiangxi Province, PR China. The strains were Gram-stain-negative, aerobic, rod-shaped, non-motile, mucoid, and yellow-pigmented. Strains dk4302 and dk4209 grew at 10–40 °C and pH 6.0–9.0, while strains xlx-73/xlx-183 grew at 15–40 °C and pH 6.0–10.0. Both strains exhibited growth in the presence of up to 3.5 % (w/v) NaCl. Phylogenetic and phylogenomic analyses based on the 16S rRNA gene sequences and 652 core genes, respectively, revealed that the four strains formed two distinct clusters in the genus . Strains dk4302 and dk4209 formed a distinct clade with XH4 and D1. The most closely related strains to xlx-73 and xlx-183 were M-SX103. The DNA G+C contents were 38.9 and 39.8 mol%. The digital DNA–DNA hybridization (dDDH) values between dk4302 and D1 and XH4 were 19.2 and 21.8 % (19.0 and 21.6 % for strain dk4209), respectively. The corresponding average nucleotide identity (ANI) values were 74.3 and 78.1 % (74.4 and 78.3 % for strain dk4209), respectively. The dDDH values between xlx-73 (xlx-183) and M-SX103 was 24.6 % (25.7 %). The corresponding ANI value was 85.7 % (85.5 % for strain xlx-183). The major fatty acid and respiratory quinone of dk4302 and xlx-73 were iso-C and MK7. The polar lipids identified in all of the novel strains were phosphatidylethanolamine, phosphoglycolipids, aminophospholipids, and phospholipids. A total of 61/190 (32.1 %) and 82/190 (43.2 %) carbon substrates were metabolized by strains dk4302 and xlx-73 in the Biolog MicroPlates, respectively. Based on the results from this polyphasic taxonomic study, two novel species in the genus are proposed, namely sp. nov. (type strain dk4302=CGMCC 1.16795=JCM 33600) and sp. nov. (type strain xlx-73=GDMCC 1.1712=JCM 33886).

  • Received:
  • Accepted:
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Keyword(s): lignocellulose degradation , novel species , phylogenetic analysis , Sphingobacterium and Tibetan gazelle
Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principal Award Recipient: JianguoXu
© 2024 The Authors
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2024-08-14
2025-11-18

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/content/journal/ijsem/10.1099/ijsem.0.006488
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Two new members of the genus Sphingobacterium: Sphingobacterium zhuxiongii sp. nov. and Sphingobacterium luzhongxinii sp. nov.
Int J Syst Evol Microbiol 74, 006488 (2024); https://doi.org/10.1099/ijsem.0.006488
/content/journal/ijsem/10.1099/ijsem.0.006488
/content/journal/ijsem/10.1099/ijsem.0.006488
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