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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 7

sp. nov., isolated from a marine alga ( sp.) No Access

Abstract

A novel Gram-stain-negative, rod-shaped, non-spore-forming, aerobic, motile bacterium with a single polar or subpolar flagellum, designated strain H3510, was isolated from marine alga collected on sea shore of Yantai, PR China. The organism grew optimally at 28 °C and pH 7.0 and in presence of 3.0 % (w/v) NaCl. The strain exhibited positive catalase activity but negative oxidase and nitrate reduction activities. The predominant cellular fatty acids were C 7 and/or C 6, 11-methyl C 7, and C. Additionally, the major polar lipids were phosphatidylglycerol, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, and phosphatidylethanolamine; the respiratory quinone was ubiquinone 10 (Q-10). The genomic DNA G+C content of strain H3510 was 54.2%. The novel strain showed the closest relationship with KMM 9699 with 98.2 % 16S rRNA gene sequence similarity. The calculated values for average nucleotide identity and DNA–DNA hybridization between strain H3510 and the phylogenetically related species were in the range of 71.3–74.9 % and 13.7–19.9 %, respectively. Based on polyphasic analyses, strain H3510 was identified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H3510 (=KCTC 8206=MCCC 1K04325). The heterologously expressed inositol 2-dehydrogenase gene from strain H3510 displayed high oxidation activity on -inositol and showed potential in the production of rare stereoisomers of inositol, such as -inositol.

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Keyword(s): 16S rRNA gene sequence , novel species , phylogenetic tree , polyphasic taxonomy and Roseibium algae
Funding
This study was supported by the:
  • the He'nan Province University Youth Researcher Support Project (Award 2016GGJS-102)
    • Principle Award Recipient: YanWang
  • the Foundation of Henan Science and Technology Agency of China (Award 202310472030)
    • Principle Award Recipient: YanWang
  • Key Laboratory of Microbial Resources and Drug Development in Guizhou Province (Award 22A350008)
    • Principle Award Recipient: TaoLi
  • the Key Scientific Research Project of Colleges and Universities in Henan Province (Award 23A180016)
    • Principle Award Recipient: YanWang
© 2024 The Authors
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2024-07-29
2025-07-12
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Roseibium algae sp. nov., isolated from a marine alga (Grateloupia sp.)
Int J Syst Evol Microbiol 74, 006475 (2024); https://doi.org/10.1099/ijsem.0.006475
/content/journal/ijsem/10.1099/ijsem.0.006475
/content/journal/ijsem/10.1099/ijsem.0.006475
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