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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 7

sp. nov., isolated from water system of Atlantic salmon () fry cultured in Chile Open Access

Abstract

Strain T-12, an orange, Gram-stain-negative, non-motile, rod-shaped strain, was isolated in November 2013 from water samples collected from an Atlantic salmon () fry culturing system at a fish farm in Chile. Phylogenetic analysis based on 16S rRNA sequences (1394 bp) revealed that strain T-12 belonged to the genus , showing close relationships to F-372 (99.48 %) and DS-20 (98.50 %). The genome size of strain T-12 was 3.28 Mb, with a G+C content of 31.1 mol%. Genome comparisons aligned strain T-12 with F-372 (GCA_011305415) and DSM 17934 (GCA_900108955). The highest digital DNA–DNA hybridization (dDDH) values were 42.6 % with F-372 (GCA_011305415) and 33.9 % with DSM 17934 (GCA_900108955). Pairwise average nucleotide identity (ANI) calculations were below the species cutoff, with the best results with F-372 being: ANIb, 90.33 %; ANIm, 91.85 %; and TETRA, 0.997 %. These dDDH and ANI results confirm that strain T-12 represents a new species. The major fatty acids were iso-C and Cω6. Detected polar lipids included phospholipids (=2), aminophospholipid (=1), aminolipid (=1) and unidentified lipids (=2). The predominant respiratory quinone was menaquinone MK7 (80 %) followed by MK-6 (20 %). Phenotypic, chemotaxonomic, and genomic data support the classification of strain T-12 (=CECT 30410=RGM 3222) as representing a novel species of , for which the name sp. nov. is proposed.

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  • Accepted:
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Keyword(s): Atlantic salmon , Chile , Flavobacterium facile and fry
Funding
This study was supported by the:
  • Agencia Nacional de Investigación y Desarrollo (Award FONDAP 1523A0007)
    • Principle Award Recipient: RubenAvendaño-Herrera
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-07-26
2025-07-15
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Flavobacterium facile sp. nov., isolated from water system of Atlantic salmon (Salmo salar) fry cultured in Chile
Int J Syst Evol Microbiol 74, 006468 (2024); https://doi.org/10.1099/ijsem.0.006468
/content/journal/ijsem/10.1099/ijsem.0.006468
/content/journal/ijsem/10.1099/ijsem.0.006468
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