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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 7

gen. nov., sp. nov. and sp. nov., isolated from desert soil No Access

Abstract

Two Gram-stain-negative, aerobic, milk-white coloured, non-motile, short rod-shaped bacteria, designated as strains SYSU D60010 and SYSU D60012, were isolated from sand samples collected from the Taklimakan Desert of Xinjiang Province in China. Both strains were positive for oxidase, catalase and nitrate reduction, but negative for amylase, HS production, hydrolysis of gelatin and cellulase. Strains SYSU D60010 and SYSU D60012 grew well at 28 °C, at pH 7 and had the same NaCl tolerance range of 0–1 % (w/v). The major fatty acids (>5 %) of strains SYSU D60010 and SYSU D60012 were summed feature 8 (C 7 and/or C 6), iso-C cyclo 8, C and iso-C 2-OH. Q-10 was the only respiratory ubiquinone. Strains SYSU D60010 and SYSU D60012 showed high 16S rRNA gene sequence similarities to SYSU M10001 (94.2 and 94.1 %), BUT-3 (92.0 and 91.9 %) and 120-1 (91.8 and 91.7 %), and the genomes were 7.4 and 5.8 Mbp in size with DNA G+C contents of 62.8 and 63.0 mol%, respectively. Phylogenetic, phenotypic and chemotaxonomic characteristics indicated that these two strains represent a novel genus and two novel species within the family . We propose the name gen. nov., sp. nov. for strain SYSU D60010, representing the type strain of this species (=KCTC 52783 =NBRC 113344) and gen. nov., sp. nov. for strain SYSU D60012, representing the type strain of this species (=KCTC 52785=NBRC 113128).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): desert soil , polyphasic taxonomy , Taklimakanibacter deserti , Taklimakanibacter lacteus and whole-genome sequencing
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32000005)
    • Principle Award Recipient: LeiDong
  • National Natural Science Foundation of China (Award 32061143043)
    • Principle Award Recipient: Wen-JunLi
  • National Natural Science Foundation of China (Award 32270076)
    • Principle Award Recipient: LeiDong
  • Guangdong Basic and Applied Basic Research Foundation (Award 2023A1515012020)
    • Principle Award Recipient: ShuaiLi
  • The Third Xinjiang Scientific Expedition Program (Award 2022xjkk1200)
    • Principle Award Recipient: LeiDong
© 2024 Sun Yat-Sen University
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Taklimakanibacter deserti gen. nov., sp. nov. and Taklimakanibacter lacteus sp. nov., isolated from desert soil
Int J Syst Evol Microbiol 74, 006462 (2024); https://doi.org/10.1099/ijsem.0.006462
/content/journal/ijsem/10.1099/ijsem.0.006462
/content/journal/ijsem/10.1099/ijsem.0.006462
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