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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 7

sp. nov. and sp. nov., isolated from soil No Access

Abstract

Two pink-pigmented bacteria, designated strains NEAU-140 and NEAU-K, were isolated from field soil collected from Linyi, Shandong Province, PR China. Both isolates were aerobic, Gram-stain-negative, rod-shaped, and facultatively methylotrophic. 16S rRNA gene sequences analysis showed that these two strains belong to the genus . Strain NEAU-140 exhibited high 16S rRNA gene sequence similarities to NBRC 15690 (97.43 %) and NBRC 105206 (97.36 %). Strain NEAU-K exhibited high 16S rRNA gene sequence similarities to NBRC 105206 (99.00 %) and DSM 23933 (98.72 %). A phylogenetic tree based on 16S rRNA gene sequences showed that strain NEAU-140 formed a clade with (95.94 %), (95.66 %) and (96.87 %), and strain NEAU-K formed a cluster with and . The predominant fatty acid in both strains was C 7. Both strains contained ubiquinone Q-10 as the only respiratory quinone. The polar lipid profiles of both strains contained diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Whole-genome phylogeny showed that strains NEAU-140 and NEAU-K formed a phyletic line with , , , , , , and . The orthologous average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain NEAU-140 and its closely related strains were lower than 82.62 and 25.90  %, respectively. The ANI and dDDH values between strain NEAU-K and its closely related strains were lower than 86.29 and 31.7 %, respectively. The genomic DNA G+C contents were 71.63 mol% for strain NEAU-140 and 69.08 mol% for strain NEAU-K. On the basis of their phenotypic and phylogenetic distinctiveness and the results of dDDH and ANI hybridization, these two isolates represent two novel species within the genus , for which the names sp. nov. (type strain NEAU-140=MCCC 1K08801=DSM 110568) and sp. nov. (type strain NEAU-K=MCCC 1K08800=DSM 110567) are proposed.

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Keyword(s): 16S rRNA gene , Methylobacterium amylolyticum sp. nov. , Methylobacterium ligniniphilum sp. nov. , phylogenetic tree , polyphasic taxonomy and whole-genome phylogeny
Funding
This study was supported by the:
  • Postdoctoral Scientific Research Development Fund of Heilongjiang Province (Award LBH-Q21072)
    • Principle Award Recipient: JunweiZhao
  • the Outstanding Youth Project of Natural Science Foundation of Heilongjiang Province (Award YQ2021C012)
    • Principle Award Recipient: JunweiZhao
  • the Research Team Project of Natural Science Foundation of Heilongjiang Province (Award TD2022C002)
    • Principle Award Recipient: WenshengXiang
  • Key Programme (Award 32030090)
    • Principle Award Recipient: WenshengXiang
  • Key Programme (Award U22A20483)
    • Principle Award Recipient: XiangjingWang
  • the National Key Research and Development Program of China (Award 2022YFD1700205)
    • Principle Award Recipient: XiangjingWang
© 2024 The Authors
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2024-07-15
2025-06-19
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Methylobacterium amylolyticum sp. nov. and Methylobacterium ligniniphilum sp. nov., isolated from soil
Int J Syst Evol Microbiol 74, 006457 (2024); https://doi.org/10.1099/ijsem.0.006457
/content/journal/ijsem/10.1099/ijsem.0.006457
/content/journal/ijsem/10.1099/ijsem.0.006457
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