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Abstract

A Gram-stain-negative, strictly aerobic, motile, flagellated, rod-shaped, halotolerant, and poly--hydroxyalkanoate-producing bacterium, designated DP4N28-3, was isolated from offshore sediment surrounding hard coral in the Dapeng peninsula (Guangdong, PR China). Growth occurred at 15–35 °C (optimal at 30 °C), pH 6.0–9.5 (optimal at 6.0–7.0), and 0.0–30.0 % NaCl concentration (w/v, optimal at 0.0–2.0 %), showing halotolerance. Phylogeny based on 16S rRNA gene sequences, five housekeeping genes, and genome sequences identified DSM 23348 (98.1 %, 16S rRNA gene sequence similarity) as the most related species to strain DP4N28-3. Average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity values between strain DP4N28-3 and DSM 23348 were all below the threshold of species demarcation. Major phenotypic differences were the flagella type and the limited sources of single carbon utilization by strain DP4N28-3, which only included acetic acid, acetoacetic acid, -glucuronic acid, and glucuronamide. Strain DP4N28-3 harboured the class I poly--hydroxyalkanoate synthase gene () and produced poly--hydroxybutyrate. The fatty acids were summed feature 8 (C 6 and/or C 7, 49.4 %) and C (13.4 %). The major cellular polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, and sulfoquinovosyl diacylglycerol. The respiratory quinone was Q-10. The results of the phylogenetic, genomic, phenotypic, and chemotaxonomic analysis indicated that the isolated strain represents the type strain of a novel species. Based on these results, strain DP4N28-3 (=MCCC 1K05639=KCTC 82803) is proposed as the type strain of the novel species sp. nov.

Funding
This study was supported by the:
  • IER Foundation 2020 (Award No. IERF202005)
    • Principle Award Recipient: Wei-shuangZheng
  • Basic and Applied Basic Research Foundation of Guangdong Province (Award 2022A1515110814)
    • Principle Award Recipient: Meng-junZhang
  • China Postdoctoral Science Foundation (Award 2022M720280)
    • Principle Award Recipient: Meng-junZhang
  • Department of Natural Resources of Guangdong Province (Award GDNRC[2022]037)
    • Principle Award Recipient: Sheng-jiLuan
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2024-09-13
2024-11-10
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