Reclassification of type strains of Rhizobium indigoferae and Sinorhizobium kummerowiae into the species Rhizobium leguminosarum and Sinorhizobium meliloti, respectively

Esther Menéndez; José David Flores-Félix; Sabhjeet Kaur; George C. diCenzo; J. Peter W. Young; Alvaro Peix; Encarna Velázquez

doi:10.1099/ijsem.0.006451

International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 7

Short Communication

Open Access

Reclassification of type strains of Rhizobium indigoferae and Sinorhizobium kummerowiae into the species Rhizobium leguminosarum and Sinorhizobium meliloti, respectively

Esther Menéndez^1,2,3, José David Flores-Félix^1,2, Sabhjeet Kaur⁴, George C. diCenzo⁴, J. Peter W. Young⁵, Alvaro Peix^3,6 and Encarna Velázquez^1,2,3
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¹ Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain ² Instituto de Investigación en Agrobiotecnología (CIALE), Universidad de Salamanca, Salamanca, Spain ³ Grupo de Interacción Planta-Microorganismo, USAL, Unidad Asociada al CSIC por el IRNASA, Salamanca, Spain ⁴ Department of Biology, Queen’s University, Kingston, Ontario, Canada ⁵ Department of Biology, University of York, York YO10 5DD, UK ⁶ Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain
*Correspondence: Alvaro Peix, [email protected]
Published: 22 July 2024 https://doi.org/10.1099/ijsem.0.006451

Abstract

The species Rhizobium indigoferae and Sinorhizobium kummerowiae were isolated from legume nodules and the 16S rRNA sequences of their respective type strains, CCBAU 71042T and CCBAU 71714T, were highly divergent from those of the other species of the genera Rhizobium and Sinorhizobium, respectively. However, the 16S rRNA gene sequences obtained for strains CCBAU 71042T and CCBAU 71714T several years after description, were different from the original ones, showing 100 % similarity to the type strains of Rhizobium leguminosarum and Sinorhizobium meliloti, respectively. Phylogenetic analyses of two housekeeping genes, recA and atpD, confirmed the high phylogenetic closeness of strains CCBAU 71042T and CCBAU 71714T to the respective type strains of R. leguminosarum and S. meliloti. In the present work, we compared the genomes of the type strains of R. indigoferae and S. kummerowiae available in several culture collections with those of the respective type strains of R. leguminosarum and S. meliloti, some of them obtained in this study. The calculated average nucleotide identity–blast and digital DNA–DNA hybridization values in both cases were higher than those recommended for species differentiation, supporting the proposal for the reclassification of the type strains of R. indigoferae and S. kummerowiae into the species R. leguminosarum and S. meliloti, respectively.

Received: 17/04/2024
Accepted: 27/06/2024
Published Online: 22/07/2024

Keyword(s): phylogeny , reclassification , Rhizobiaceae , Rhizobium indigoferae , Rhizobium leguminosarum , Sinorhizobium kummerowiae and Sinorhizobium meliloti

Funding

This study was supported by the:

Natural Sciences and Engineering Research Council of Canada (Award RGPIN-2020-07000)
- Principle Award Recipient: GeorgeC diCenzo
H2020 Marie Skłodowska-Curie Actions (Award 101034371)
- Principle Award Recipient: JoséDavid Flores-Félix
H2020 Marie Skłodowska-Curie Actions (Award 897795)
- Principle Award Recipient: EstherMenéndez
Junta de Castilla y León (Award CLU-2019-05)
- Principle Award Recipient: AlvaroPeix
Junta de Castilla y León (Award CLU-2O18-04)
- Principle Award Recipient: EncarnaVelázquez

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Reclassification of type strains of Rhizobium indigoferae and Sinorhizobium kummerowiae into the species Rhizobium leguminosarum and Sinorhizobium meliloti, respectively

Int J Syst Evol Microbiol 74, 006451 (2024); https://doi.org/10.1099/ijsem.0.006451

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Supplements

International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 7

Short Communication

Open Access

Reclassification of type strains of Rhizobium indigoferae and Sinorhizobium kummerowiae into the species Rhizobium leguminosarum and Sinorhizobium meliloti, respectively

Abstract

Funding

Supplementary material 1

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