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Abstract

A novel facultatively anaerobic and Gram-stain-negative bacterium, designated FJH33, was isolated from mangrove sediment sampled in Zhangzhou, PR China. Cells of strain FJH33 were rod-shaped or slightly curved-shaped, with widths of 0.3–0.5 µm and lengths of 1.0–3.0 µm. Optimum growth of strain FJH33 occurred in the presence of 3 % NaCl (w/v), at 33 °C and at pH 7.0. Oxidase activity was negative, while catalase activity was positive. Its iron-reducing ability was determined. Based on 16S rRNA gene sequence similarity, strain FJH33 was most closely related to XSD2 (95.1 %), followed by Y2-1-60 (95.0 %) and 5E3 (94.9 %). Genome analysis of strains FJH33 and XSD2 revealed low genome relatedness, with an average nucleotide identity value of 73.8% and a digital DNA–DNA hybridization value of 19.0%. Phylogenetic trees built from 16S rRNA genes and genome sequences showed that strain FJH33 represents a relatively independent phylogenetic lineage within the genus . The major cellular fatty acids (≥10 %) were iso-C and C 9. The sole respiratory quinone was MK-7. The polar lipids consisted of phosphatidylethanolamine, diphosphatidylcholine, diphosphatidyglycerol and one unidentified lipid. The DNA G+C content was 41.4 mol%. Based on the integrated results of phylogenetic, physiological, biochemical and chemotaxonomic characterizations, we propose that strain FJH33 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJH33 (=KCTC 102210=MCCC 1H01459).

Funding
This study was supported by the:
  • Science and Technology Fundamental Resources Investigation Program (Award 2022FY101100)
    • Principle Award Recipient: Zong-JunDu
  • National Natural Science Foundation of China (Award 92351301, 32070002)
    • Principle Award Recipient: Zong-JunDu
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2024-07-12
2025-07-12
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