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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 74, Issue 7

gen. nov., sp. nov., isolated from intertidal sediment, and genomic comparison with all genera in the family No Access

Abstract

A novel slightly halophilic, aerobic, and Gram-stain-negative strain, designated as CH-27, was isolated during a bacterial resource investigation of intertidal sediment collected from Xiaoshi Island in Weihai, PR China. Cells of strain CH-27 were rod-shaped with widths of 0.3–0.6 µm and lengths of 2.0–11.0 µm. Strain CH-27 grew optimally at 37 °C, pH 7.0 and with 2.0 % (w/v) NaCl. Catalase activity was weakly positive and oxidase activity was positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CH-27 was most related to KCTC 92639 (93.6 %), followed by MCCC 1K00261 (92.0 %). Based on genome comparisons between strain CH-27 and KCTC 92639, the average amino acid identity was 63.6 % and the percentage of conserved proteins was 48.3 %. The major cellular fatty acid of strain CH-27 (≥10 %) was iso-C and the sole respiratory quinone was quinone-8. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, and aminophospholipid. The DNA G+C content was 62.7 mol%. Based on comprehensive analysis of its phylogenetic, physiological, biochemical, and chemotaxonomic characteristics, strain CH-27 represents a novel species in a novel genus, for which the name gen. nov., sp.nov. is proposed. The type strain is CH-27 (=MCCC 1H00480=KCTC 8011).

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Keyword(s): comparative genomic analysis , Elongatibacter sediminis , intertidal sediment , polyphasic taxonomy and Wenzhouxiangellaceae
Funding
This study was supported by the:
  • Basic and Applied Basic Research Foundation of Guangdong Province (Award 2022A1515110773)
    • Principle Award Recipient: Meng-QiYe
  • National Natural Science Foundation of China (Award 32200003)
    • Principle Award Recipient: Meng-QiYe
  • Natural Science Foundation of Shandong Province (Award ZR2022QC106)
    • Principle Award Recipient: Meng-QiYe
© 2024 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006440
2024-07-03
2025-06-18
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/content/journal/ijsem/10.1099/ijsem.0.006440
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Elongatibacter sediminis gen. nov., sp. nov., isolated from intertidal sediment, and genomic comparison with all genera in the family Wenzhouxiangellaceae
Int J Syst Evol Microbiol 74, 006440 (2024); https://doi.org/10.1099/ijsem.0.006440
/content/journal/ijsem/10.1099/ijsem.0.006440
/content/journal/ijsem/10.1099/ijsem.0.006440
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