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Abstract

Two Gram-stain-positive, aerobic, oxidase- and catalase-negative, non-motile, and short rod-shaped actinomycetes, named SYSU T00b441 and SYSU T00b490, were isolated from tidal flat sediment located in Guangdong province, PR China. The 16S rRNA gene sequence similarity, average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between SYSU T00b441 and SYSU T00b490 were 99.3, 99.5 and 97.1 %, respectively. Strains SYSU T00b441 and SYSU T00b490 exhibited the highest 16S rRNA gene sequence similarities to CF 5-4 (97.1 %/98.2 %), with ANI values of 74.01/73.88 % and dDDH values of 20.5/20.4 %. In the phylogenomic tree, the two isolates were affiliated with the genus . The genomes of strains SYSU T00b441 and SYSU T00b490 were 3.31 and 3.34 Mb, and both had DNA G+C contents of 72.8 mol%, coding 3077 and 3085 CDSs, three and three rRNA genes, and 53 and 51 tRNAs, respectively. Growth occurred at 15–40 °C (optimum, 28–30 °C), pH 4.0–10.0 (optimum, 7.0) and in the presence of 0–7 % (w/v) NaCl (optimum, 3 %). The major fatty acids (>10  %) of strains SYSU T00b441 and SYSU T00b490 were anteiso-C and C. The major respiratory quinone was identified as MK-10(H). The polar lipids of strains SYSU T00b441 and SYSU T00b490 were diphosphatidyl glycerol, phosphatidylglycerol, phosphoglycolipid, phosphatidyl ethanolamine, two phosphatidylinositol mannosides, two glycolipids and two phospholipids. Based on these data, the two strains (SYSU T00b441 and SYSU T00b490) represent a novel species of the genus , for which the name sp. nov is proposed. The type strain is SYSU T00b441 (=GDMCC 1.3827=KCTC 49943).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32000005)
    • Principle Award Recipient: LeiDong
  • National Natural Science Foundation of China (Award 32270076)
    • Principle Award Recipient: LeiDong
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2019FY100700)
    • Principle Award Recipient: Bao-ZhuFang
  • Key-Area Research and Development Program of Guangdong Province (Award 2022B0202110001)
    • Principle Award Recipient: Wen-JunLi
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2024-06-19
2024-07-15
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