1887

Abstract

A Gram-stain-positive, rod-shaped, aerobic, motile bacterium, J379, was isolated from radioactive water spring C1, located in a former silver–uranium mine in the Czech Republic. This slow-growing strain exhibited optimal growth at 24–28 °C on solid media with <1 % salt concentration and alkaline pH 8–10. The only respiratory quinone found in strain J379 was MK-7(H). C ω9 (60.9 %), C (9.4 %), C and alcohol-C (both 6.2 %) were found to be the major fatty acids. The peptidoglycan contained directly cross-linked -diaminopimelic acid. Phylogenetic reconstruction based on the 16S rRNA gene sequences and the core-genome analysis revealed that strain J379 forms a separate phylogenetic lineage within the recently amended order . A comparison of the 16S rRNA gene sequences between strain J379 and other members of the order showed <96 % similarity. This analysis revealed that the closest type strains were D16/0 /H6 (95.2 %), 0166_1 (94.9 %) and KV-962 (94.5 %). Whole-genome analysis showed that the closest type strain was BR7-21 with an average nucleotide identity of 78 %, average amino acid identity of 63.2 % and percentage of conserved proteins of 48.2 %. The G+C content of the J379 genomic DNA was 71.7 mol%. Based on the phylogenetic and phylogenomic data, as well as its physiological characteristics, strain J379 is proposed to represent a type strain (DSM 113746=CCM 9300) of gen. nov. sp. nov. within the family .

Funding
This study was supported by the:
  • Ministerstvo Obrany České Republiky (Award 907930101413)
    • Principle Award Recipient: PetrPajer
  • Ministerstvo Školství, Mládeže a Tělovýchovy (Award LM2023055)
    • Principle Award Recipient: OndrejUhlik
  • Grantová Agentura České Republiky (Award 18-00036S)
    • Principle Award Recipient: OndrejUhlik
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2024-06-26
2024-07-15
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